Comparative studies of protein production in liver and muscle between commercial broiler and indigenous chicken /
Rapid juvenile growth among broilers may induce health defects including stress and metabolic diseases that can alter the expression of the protein in various parts of the body. In this study, the differences in protein expression from chicken liver and muscle between fast-growing commercial broiler...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
Kuantan, Pahang :
Kulliyyah of Science, International Islamic University Malaysia,
2016
|
| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| LEADER | 042760000a22002890004500 | ||
|---|---|---|---|
| 008 | 160613s2016 my a f m 000 0 eng d | ||
| 040 | |a UIAM |b eng |e rda | ||
| 041 | |a eng | ||
| 043 | |a a-my--- | ||
| 050 | 0 | 0 | |a QP551 |
| 100 | 0 | |a Muhamad Shafiq bin Abu Hassan, |e author | |
| 245 | 1 | |a Comparative studies of protein production in liver and muscle between commercial broiler and indigenous chicken / |c by Muhamad Shafiq bin Abu Hassan | |
| 264 | 1 | |a Kuantan, Pahang : |b Kulliyyah of Science, International Islamic University Malaysia, |c 2016 | |
| 300 | |a xiii, 114 leaves : |b illustrations ; |c 30cm. | ||
| 336 | |2 rdacontent |a text | ||
| 502 | |a Thesis (MSBTS)--International Islamic University Malaysia, 2016. | ||
| 504 | |a Includes bibliographical references (leaves 72-85). | ||
| 520 | |a Rapid juvenile growth among broilers may induce health defects including stress and metabolic diseases that can alter the expression of the protein in various parts of the body. In this study, the differences in protein expression from chicken liver and muscle between fast-growing commercial broilers (CB) and slow-growing indigenous chicken (IC) have been analyzed using SDS-PAGE and 2D-PAGE. In the liver, six spots were successfully identified differentially expressed in term of intensity. Those proteins are phosphoenolpyruvate carboxykinase (PEPCK) (2 spots), delta-1-pyrolline-5-carboxylate (P5CDH), soluble epoxide hydrolase (sEH), heat shock protein 90 (HSP90) and actin. PEPCK and P5CDH enzymes were expressed higher in CB compared to IC, suggesting the utilization of alternative metabolic pathways for broilers' energy metabolism. The sEH expression was found higher in CB compared to IC indicating the process of xenobiotic metabolic activity to eliminate harmful epoxide inside CB liver. HSP90 was found less in CB compared to IC, which might indicate the lack of regulation of heat stress response in CB. The higher expression of actin in IC liver has suggested a good form of structure integrity and good management of bile flows facilitation in IC liver. All of these findings showed that IC might have a better liver and metabolic activity compared to CB. In the muscle, six differentially expressed spots between both samples were successfully identified. Those six proteins are alpha-enolase, pyruvate kinase, heat shock 70 kDa protein, stress-induced phosphoprotein, myosin regulatory light chain and myozenin. In general, it is suggested that CB may suffer from hypoxia or prolong exercise, which was shown through the shifted metabolic activity from aerobic towards anaerobic metabolism. Moreover, CB muscle was likely to utilize 'glucose-compensation' pathways to fuel the cellular after glucose deprivation. It is also found that CB may endure from heat stress compared to IC. In term of meat quality, CB muscle has a lack of structural proteins composition compared to IC. It might be suggested that CB meat is tender compared to IC due to the lack of this structural proteins. To summarize, it is found that there are differences in protein expression between slow-growing IC with the fast-growing CB in liver and muscle. The proteins have demonstrated differently in energy metabolism and stress regulation as well as structural proteins in CB compared to IC. This current discovery will be a useful tools to uncover the molecular basis of physiological differences in liver and muscle that undergo rapid growth, metabolic syndromes and stress response. | ||
| 596 | |a 1 6 | ||
| 655 | 7 | |a Theses, IIUM local | |
| 690 | |a Dissertations, Academic |x Department of Biotechnology |z IIUM | ||
| 710 | 2 | |a International Islamic University Malaysia. |b Department of Biotechnology | |
| 856 | 4 | |u https://lib.iium.edu.my/mom/services/mom/document/getFile/lrHXtoMsOlzsqtnvTad5edOL3iR9imHO20161011145553264 |z Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. | |
| 900 | |a sbh-aaz-ls | ||
| 999 | |c 435992 |d 469151 | ||
| 952 | |0 0 |6 T QP 000551 M9522C 2016 |7 0 |8 THESES |9 761118 |a KIMC |b KIMC |c CLOSEACCES |g 0.00 |o t QP 551 M9522C 2016 |p 11100346737 |r 2019-09-04 |t 1 |v 0.00 |y THESIS | ||
| 952 | |0 0 |6 TS CDF QP 551 M9522C 2016 |7 0 |8 THESES |9 854644 |a IIUM |b IIUM |c MULTIMEDIA |g 0.00 |o ts cdf QP 551 M9522C 2016 |p 11100346738 |r 2018-04-05 |t 1 |v 0.00 |y THESISDIG | ||