Effects of Tualang Honey on non-alcoholic steatohepatitis in rat model /
Non-alcoholic Steatohepatitis (NASH) is a common chronic liver disease that may progress to cirrhosis and hepatocellular carcinoma. Currently, there is limited therapy available. Tualang honey which is known for its anti-inflammatory and anti-oxidative effects may improve NASH as inflammatory proces...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
Kuantan, Pahang :
Kulliyyah of Medicine, International Islamic University Malaysia,
2016
|
| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| LEADER | 047010000a22003490004500 | ||
|---|---|---|---|
| 008 | 170223t2016 my a g m 000 0 eng d | ||
| 040 | |a UIAM |b eng | ||
| 041 | |a eng | ||
| 043 | |a a-my--- | ||
| 050 | 0 | 0 | |a RC848.F3 |
| 100 | 0 | |a Azril Shahreez bin Abdul Ghani | |
| 245 | 1 | |a Effects of Tualang Honey on non-alcoholic steatohepatitis in rat model / |c by Azril Shahreez bin Abdul Ghani | |
| 260 | |a Kuantan, Pahang : |b Kulliyyah of Medicine, International Islamic University Malaysia, |c 2016 | ||
| 300 | |a xxi, 139 leaves : |b ill. ; |c 30cm. | ||
| 500 | |a Abstracts in English and Arabic. | ||
| 500 | |a "A thesis submitted in fulfilment of the requirement for the degree of Master of Medical Sciences." --On t. p. | ||
| 502 | |a Thesis (MMDSc)--International Islamic University Malaysia, 2016. | ||
| 504 | |a Includes bibliographical references (leaves 125-137). | ||
| 520 | |a Non-alcoholic Steatohepatitis (NASH) is a common chronic liver disease that may progress to cirrhosis and hepatocellular carcinoma. Currently, there is limited therapy available. Tualang honey which is known for its anti-inflammatory and anti-oxidative effects may improve NASH as inflammatory process and oxidative damage have been implicated in the pathogenesis. However studies utilizing animal models induced with cholesterol diet ranging from 0.5 – 2% are hampered by inconsistent yield of NASH features as assessed histologically. The aims of this study are to establish NASH animal model utilizing 12% cholesterol diet and to investigate the effects of Tualang honey on NASH. This study is divided into 2 phases: the induction phase with cholesterol diet and the treatment phase with Tualang honey. In the induction phase, 42 Sprague-Dawley rats were randomly divided into standard diet (std) group (n=12) and NASH group (n=30). The 12 rats in the std group were fed with commercial rat pellet while the 30 rats in the NASH group were fed with 1% cholesterol (1% CD) diet for 14 weeks. The experiment was extended for an additional 6 weeks with increment of cholesterol in the diet from 1% to 12 % in the NASH group since the liver histology did not show features of NASH in the 1% CD. At the end of the additional 6 weeks period, liver histology showed NASH changes in the 12% CD. Subsequently in the treatment phase, the rats in the NASH group were randomly divided and continued on the cholesterol diet in addition to administration of 0.2, 1.2, 2.4 and 3.0 g of Tualang honey per kg body weight daily respectively as treatment for 4 weeks. Results of the histological sections of the livers of the 12% CD rats in the induction phase exhibited features consistent with NASH which include macrovesicular steatosis, occasional hepatocyte ballooning degeneration with mild to moderate lobular and portal inflammation. The 12% CD group revealed significantly increased serum alanine aminotransferase (ALT), serum insulin levels, homeostatic model assessment insulin resistance (HOMA-IR), serum total cholesterol (TC), serum low-density lipoprotein cholesterol (LDL-c) and reduced high-density lipoprotein cholesterol (HDL-c) in rats. Tualang honey supplementation in the treatment groups showed significant improvement in the histological grading and non-alcoholic fatty liver disease (NAFLD) activity scores. Tualang honey also significantly reduced serum ALT, serum insulin, HOMA-IR, serum LDL-c and increased HDL-c in NASH rats in these groups. In conclusion, this study has successfully induced mild – moderate NASH rat model utilizing 12% cholesterol diet and also demonstrated that Tualang honey administration improved histological grading, NAFLD activity score, insulin resistance, liver function and and dyslipidemia in NASH animal model. | ||
| 596 | |a 1 6 | ||
| 650 | 0 | |a Fatty liver | |
| 650 | 0 | |a Fatty liver |x Treatment | |
| 650 | 0 | |a Liver |x Diseases | |
| 650 | 0 | |a Honey |x Therapeutic use | |
| 655 | 7 | |a Theses, IIUM local | |
| 690 | |a Dissertations, Academic |x Department of Basic Medical Sciences |z IIUM | ||
| 710 | 2 | |a International Islamic University Malaysia. |b Department of Basic Medical Sciences | |
| 856 | 4 | |u https://lib.iium.edu.my/mom/services/mom/document/getFile/Egyozal2QwuSvXHy0BadWJp5eGPV9JQp20160928113211875 |z Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. | |
| 900 | |a sbh-ro | ||
| 999 | |c 436108 |d 468063 | ||
| 952 | |0 0 |6 T RC 000848 F3 A997E 2016 |7 0 |8 THESES |9 761090 |a KIMC |b KIMC |c CLOSEACCES |g 0.00 |o t RC 848 F3 A997E 2016 |p 11100346691 |r 2019-09-04 |t 1 |v 0.00 |y THESIS | ||
| 952 | |0 0 |6 TS CDF RC 848 F3 A997E 2016 |7 0 |8 THESES |9 854612 |a IIUM |b IIUM |c MULTIMEDIA |g 0.00 |o ts cdf RC 848 F3 A997E 2016 |p 11100346692 |r 2017-10-26 |t 1 |v 0.00 |y THESISDIG | ||