Cloning of phytase from Bacillus subtilis ASUIA243 in Escherichia Coli and Pichia pastoris system /
Phytase gene from Bacillus subtilis ASUIA243 was amplified with specific primers for Escherichia coli and Pichia pastoris transformation. The amplified blunt-ended gene was cloned into cloning vector pCR®-Blunt II-TOPO® and transformed into Escherichia coli TOPlO competent cells, while the sticky-en...
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| 格式: | Thesis |
| 语言: | English |
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Kuala Lumpur:
Kulliyyah of Engineering,International Islamic University Malaysia,
2012
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