Pilot scale production and immobilization of lipase using palm oil mill effluent as basal medium /
Lipase recognized as one of the most important enzymes which has widely industrial uses. Production of low cost enzyme using potential raw material will significantly enhance and attractive to the enzyme market. A local residue of palm oil mill effluent (POME) abundantly found and can be used as a b...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur:
Kulliyyah of Engineering, International Islamic University Malaysia,
2014
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | Lipase recognized as one of the most important enzymes which has widely industrial uses. Production of low cost enzyme using potential raw material will significantly enhance and attractive to the enzyme market. A local residue of palm oil mill effluent (POME) abundantly found and can be used as a basal medium for lipase production. The objectives of this study are to develop a scale up strategy of lipase production (300L of stirred tank bioreactor), immobilized the produced lipase and characterized it for industrial uses. The lipase production was carried out by liquid state bioconversion of POME whereas Candida cylindracea as the microorganism. The fermentation media composition was based on the optimization from the previous study with 1% total suspended solid (TSS) based POME media, 0.5% (w/v) peptone, 0.7% (v/v) Tween-80 and 2.2% inoculums. The fermentation was carried out for 48 hours at temperature of 30°C, pH value 6 and aeration at 0.5 volume per volum per minute (vvm). The results showed that the maximum lipase production 5.72 U/ml and 21.34 U/ml was determined with the scale up strategy of tip speed (agitation: 98 rpm) and P/V constant (130 rpm) respectively. The extracelular enzyme from bioreactor production was purified with cross flow filtration and used for immobilization. Four accesible support materials were screened for their potential used in immobilization. The most suitable support material was activated carbon which immobilized lipase up to 94%. Sequential optimization strategy based on statistical experimental design including one-factor-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by response surface methodology (RSM) based on the face-centered central composite design (FCCCD). On statistical analysis of the results, the optimum enzyme concentration loading, agitation rates and activated carbon concentration were found to be 30U/ml, 300 rpm and 8 g/L respectively, with a maximum immobilization activity of 3732.9 U/g after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high coeficient of (R2) value of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05. The temperature and pH stability of the immobilized lipase were found to be more stable. It could still retain in a high enzyme activity up to 65oC and active in range pH 6 to pH 8. Among the buffers the enzyme desorption was best in phosphate buffer pH 8. The application of the enzyme through sludge palm oil hydroliysis noted that 60.12 % and 66.42% of the hydrolysis toward lipid was achieved with free and immobilized lipases respectively at 24 h, pH 8, and temperature 45°C. The findings of this study showed that pilot plant lipase production was possible using two proposed strategies provided and its improvement characteristic through activated carbon immobilization could be one of the contributions of POME bioconversion into valuable product. |
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| Physical Description: | xiii, 125 leaves : ill. ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 91-115). |