Process optimization for astaxanthin extraction from Malaysian shrimp waste using lactobacillus rhamnosus fermentation /
Astaxanthin is a red-orange pigment that belongs to the family of the carotenoids. Astaxanthin is mainly used for animal feed in aquaculture where it is commonly produced synthetically at a very high cost. It is estimated that Malaysia spends annually RM 80-100 millions to import astaxanthin for aqu...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur :
Kulliyyah of Engineering, International Islamic University Malaysia,
2013
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| Subjects: | |
| Online Access: | http://studentrepo.iium.edu.my/handle/123456789/5117 |
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| Summary: | Astaxanthin is a red-orange pigment that belongs to the family of the carotenoids. Astaxanthin is mainly used for animal feed in aquaculture where it is commonly produced synthetically at a very high cost. It is estimated that Malaysia spends annually RM 80-100 millions to import astaxanthin for aqua farming industries. Yet, Malaysia is rich in natural resources of astaxanthin, especially those from marine origin such as fish, red algae, molluscs and shrimp. Hence, the aim of this paper is to extract astaxanthin from the waste of shrimp (Penaeus merguiensis) of Malaysia origin via fermentation process using Lactobacillus rhamnosus and optimize the media fermentation composition by using alternative carbon sources from agriculture wastes. In this paper, seven carbon sources namely glucose, sucrose, citric acid, empty fruit bunch, jack fruit peel, banana peel and cassava peel; had been screened as media component for fermentation process using Plackett Burman screening design experiment. One-Factor-at-A-Time (OFAT) method and response surface methodology (RSM) were also employed to optimize the medium components such as, inoculum size, glucose and banana peel concentrations. The findings of this work showed that astaxanthin can be extracted from shrimp waste through Lactobacillus rhamnosus fermentation. Six out of these seven substrates had the potential to extract astaxanthin, however, the combination of glucose and banana peel gave the highest yield of astaxanthin with 63.93 μg/g of the waste compared to 30.62 μg/g from the control (no substrate). The maximum astaxanthin yield (78.08 μg/g) was obtained from an optimized media consisting of (5%) inoculum size, (5%) glucose concentration and (15%) banana peel concentration. Further studies were suggested to optimize process condition such as temperature, pH, agitation speed and CO2 concentration in order to achieve higher astaxanthin yield. |
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| Physical Description: | xiii, 83 leaves : ill. ; 30cm. |
| Bibliography: | Includes bibliographic references (leaves 74-83). |