Modification and characterization of phytases for animal feed production /
Phytases catalyze the hydrolysis phytic acid and are able to improve the nutritional quality of phytate rich diet. Monogastric animal such as poultry and fish have lack of significant activity to hydrolyze phytate that contributes to elimination of beneficial nutrient for growth and therefore, contr...
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| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur :
Kulliyyah of Engineering, International Islamic University Malaysia,
2012
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| LEADER | 042560000a22004330004500 | ||
|---|---|---|---|
| 008 | 130307s2012 my a f m 000 0 eng d | ||
| 040 | |a UIAM |b eng |e rda | ||
| 041 | |a eng | ||
| 043 | |a a-my--- | ||
| 050 | 0 | 0 | |a TP248.65.E59 |
| 100 | 0 | |a Nurhusna Binti Samsudin, |e author | |
| 245 | 1 | 0 | |a Modification and characterization of phytases for animal feed production / |c by Nurhusna Binti Samsudin |
| 264 | 1 | |a Kuala Lumpur : |b Kulliyyah of Engineering, International Islamic University Malaysia, |c 2012 | |
| 300 | |a xvii, 142 leaves : |b illustrations ; |c 30cm. | ||
| 336 | |2 rdacontent |a text | ||
| 337 | |2 rdamedia |a unmediated | ||
| 337 | |2 rdamedia |a computer | ||
| 338 | |2 rdacarrier |a volume | ||
| 338 | |2 rdacarrier |a computer disc | ||
| 338 | |2 rdacarrier |a online resource | ||
| 347 | |2 rdaft |a text file |b PDF | ||
| 500 | |a Abstracts in English and Arabic. | ||
| 500 | |a " A thesis submitted in fulfilment of the requirement for the degree of Master of Science in Biotechnology Engineering."--On title page. | ||
| 502 | |a Thesis (MSBTE)--International Islamic University Malaysia, 2012. | ||
| 504 | |a Includes bibliographical references (leaves 125-134) | ||
| 520 | |a Phytases catalyze the hydrolysis phytic acid and are able to improve the nutritional quality of phytate rich diet. Monogastric animal such as poultry and fish have lack of significant activity to hydrolyze phytate that contributes to elimination of beneficial nutrient for growth and therefore, contribute to land pollution, eutrophication of ground water and aquatic environment. In addition, it leads to the negative effect on vitamin utilization that leads to the emaciation, retarded growth and reproductive failure to animals. Due to the importance of microbial sources for the commercial production of phytases, a waste water bacterium phytase is the subject of interest in this work. In silica experiment is used to identify and examine the active site of waste water bacterium phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the ligand binding strength. From the directive results of computational studies, point mutation was introduced by site directed mutagenesis using polymerase chain reaction (PCR). Mutagenesis is achieved by two step PCR megaprimer procedure. After mutants of PhyFAUIAl were generated, variants were expressed in Escherichia coli (DH5a) and characterized for improved catalytic properties. Detailed studies on kinetic stability and the thermostability of the mutational variants M2 l 6R, E2 l 9R and A 116T, and the respective multiple mutants M216R/E219R and M216R/E2 l 9R/F254E are presented. The selected substitutions at position M216R and A 116T improved the catalytic activity relative to the wild type enzyme. Thermostability study showed that variant M216R showed over 28% improvement in thermostability (90°C for 30 minutes) and 6.2°C increased in melting temperature (T111) over wild type en:z;yme. Structural predictions suggest that substitution of M216R might introduce additional hydrogen bonds with adjacent residues, improving the enzymes thermostability by stabilizing local interaction. | ||
| 596 | |a 1 | ||
| 650 | 0 | |a Microbial enzymes |x Biotechnology | |
| 650 | 0 | |a Phytases | |
| 650 | 0 | |a Enzymes in animal nutrition | |
| 655 | 7 | |a Theses, IIUM local | |
| 690 | |a Dissertations, Academic |x Department of Biotechnology Engineering. |z IIUM | ||
| 710 | 2 | |a International Islamic University Malaysia. |b Department of Biotechnology Engineering. | |
| 856 | |u http://studentrepo.iium.edu.my/handle/123456789/5002 |z Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. | ||
| 900 | |a sbh-aaz-naw | ||
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| 999 | |c 438288 |c 438288 |d 468999 |d 468999 | ||
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