Study of the potential activity of aqueous extract of trigonella foenum-graecum (fenugreek) seeds on ovarian superovulation in female rats /
Ovulation induction medications and the assisted reproductive technology (ART) have been used since long time for treatment of the anovulation disorders, which represent about 30% of infertility cases. However, the increase of the incidence of ovulation induction and ART, causes increase of multiple...
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuantan, Pahang :
Kulliyyah of Medicine, International Islamic University Malaysia,
2015
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | Ovulation induction medications and the assisted reproductive technology (ART) have been used since long time for treatment of the anovulation disorders, which represent about 30% of infertility cases. However, the increase of the incidence of ovulation induction and ART, causes increase of multiple pregnancy and ovarian hyper-stimulation syndrome as two main complications. These two complications are caused by hyper-stimulation of ovaries. Trigonella foenum-graecum (fenugreek) is an herbal plant that has been studied for its anti-fertility activity. The aim of this research was to study the potential activity of fenugreek seed aqueous extract (FAE) on ovarian superovulation in female rats by observing the relative weight of ovaries, quantifying the ovarian follicles (secondary, antral and atretic follicles) and determining serum estradiol concentrations. Twenty four cyclic Sprague Dawley female rats, 7 – 8 week-age, were divided into three groups (A, B and C). Group A considered as control group, the female rats in group B and C treated with fenugreek seed aqueous extract 500mg/kg and 1000mg/kg respectively. The extract was administering orally into rats in treated groups for 14 days. Vaginal smears were taken and examined daily to determine the different phases of estrous cycle. During diestrus phase of estrous cycle, the female rats in the three groups were treated with subcutaneous injection of decreasing doses of recombinant human FSH (rhFSH) to induce the superovulation. In next day (proestrus phase), the rats were anesthetized and blood samples were collected by retro-orbital technique to estimate the estradiol levels. Then, both ovaries of each rat were removed for histological examination. Both doses of FAE have not shown significant reduction in relative ovarian weight (p-value = 0.233). However, administering of 500 mg/kg and 1000mg/kg body weight of the extract caused significant reduction in the number of secondary follicles (p-value < 0.005), antral follicles (p-value < 0.005) and corpora lutea (p-value < 0.005), while the number of atretic follicles was increased significantly (p-value < 0.005) in both treated groups compared to control group. Moreover, the atretic follicle number was significantly (p-value = 0.01) higher in group C (treated with 1000mg/kg of FAE) compared to group B (treated with 500mg/kg of FAE). The serum estradiol concentrations were significantly decreased in both treated groups compared to control group (p-value < 0.05). As an outcome of this study, FAE showed an effect on ovarian superovulation by decreasing the serum estradiol concentrations, reducing the number of secondary and antral follicles as well as the number of corpora lutea, and increasing the atretic follicle number. |
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| Physical Description: | xiv, 62 leaves : ill. ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 56-62). |