Screening of the potential antioxidant, antimicrobial and antiproliferative activities of Donax Grandis /
In this study, crude extracts of different parts (leaves, fruits, roots and stems) of Donax grandis using different types of organic solvents (hexane, dichloromethane and methanol) and water extracts were evaluated by performing phytochemicals screening and proximate analysis of saponins. The methan...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur :
Kulliyyah of Science, International Islamic University Malaysia,
2013
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| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| LEADER | 041590000a22002770004500 | ||
|---|---|---|---|
| 008 | 130715t2013 my a g m 000 0 eng d | ||
| 040 | |a UIAM |b eng | ||
| 041 | |a eng | ||
| 043 | |a a-my--- | ||
| 050 | 0 | 0 | |a QK861 |
| 100 | 0 | |a Husna Hawa Mohd Hassan | |
| 245 | 1 | |a Screening of the potential antioxidant, antimicrobial and antiproliferative activities of Donax Grandis / |c by Husna Hawa Mohd Hassan | |
| 260 | |a Kuala Lumpur : |b Kulliyyah of Science, International Islamic University Malaysia, |c 2013 | ||
| 300 | |a xvii, 121 leaves : |b ill ; |c 30cm. | ||
| 502 | |a Thesis (MSBTS)--International Islamic University Malaysia, 2013. | ||
| 504 | |a Includes bibliographical references (leaves 99-111). | ||
| 520 | |a In this study, crude extracts of different parts (leaves, fruits, roots and stems) of Donax grandis using different types of organic solvents (hexane, dichloromethane and methanol) and water extracts were evaluated by performing phytochemicals screening and proximate analysis of saponins. The methanolic crude extracts of D. grandis were further evaluated for antioxidant, antimicrobial and antiproliferative activities. Phytochemical screening of the crude extracts (leaves, fruits, stems and roots) revealed the presence of phenolic compounds, alkaloids, tannins, phytosterols, cardiac glycosides, terpenoids, steroids, saponins and flavonoids. The results showed that the fruits contained the highest amount of saponins (2.39 wt. %). Total phenolic content (TPC) which was determined according to the Folin-Ciocalteu methods showed that all parts of the plant samples had low total phenolic content ranged from 0.18 to 0.65 mg GAE/g extract, with fruit having the highest phenolic content. Free radical scavenging activity of the methonolic crude extracts were measured by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, and the IC50 value of the leaf, fruit, stem and root extracts ranged from 14.86 to 21.85 mg/mL. Antimicrobial activity using disc diffusion method on methanolic crude extracts at concentration of 40 μg/disc were evaluated against two Gram-positive bacteria, two Gram-negative bacteria and three fungi strains. The extracts showed significant antimicrobial activities against bacteria and fungi strains. The inhibition zones ranged from 7.00 ± 0.57 mm – 18.00 ± 0.5 mm, whereas MIC values ranged from 6.25 μg/mL – 100 μg/mL. 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay was used to screen for anticancer activity of the respective D. grandis extracts, at various concentrations, against human lung carcinoma (H1299) and human oral tumor (HSC-3) cell lines. The methanolic extracts of fruits and roots proved to have medium inhibition with the IC50 values of 28.3 ± 2.9 μg/mL and 273.3 ± 15.3 μg/mL for H1299, and 520.0 ± 36.1 μg/mL and 583.3 ± 12.3 μg/mL for HSC-3 respectively. The isolation and structural elucidation of a pure compound from methanolic extract of D. grandis' fruits revealed one potent antiproliferative compound, i.e. (25R)-Spirost-5-en-3beta-ol; 3b-hydroxy-5-spirostene, also known as diosgenin which collectively responsible for unleashing the antiproliferative activity against both H1299 and HSC-3 cell lines. The finding, vividly support its traditional claims and form a strong basis to explore D. grandis anticancer properties. | ||
| 596 | |a 1 6 | ||
| 655 | 7 | |a Theses, IIUM local | |
| 690 | |a Dissertations, Academic |x Department of Biotechnology Science |z IIUM | ||
| 710 | 2 | |a International Islamic University Malaysia. |b Department of Biotechnology Science | |
| 856 | 4 | |u https://lib.iium.edu.my/mom/services/mom/document/getFile/B6joMRzEzrGdHQhBN6paktHQNOBTAVYv20140404111935386 |z Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. | |
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| 952 | |0 0 |6 TS CDF QK 861 H971S 2013 |7 0 |8 THESES |9 851100 |a IIUM |b IIUM |c MULTIMEDIA |g 0.00 |o ts cdf QK 861 H971S 2013 |p 00011288998 |r 2017-10-26 |t 1 |v 0.00 |y THESISDIG | ||