Identification of chemical parameters for the extraction of Nahar (Mesua ferrea) seed oil for antibacterial activities) /
Prior research studies on antibacterial agent and water disinfectants have revealed some problems associated with synthetic materials. Also, the search for new antimicrobial agents and natural disinfectants make different plant species and parts suitable for this purpose. In this research, various p...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur:
Kulliyyah of Engineering, International Islamic University Malaysia,
2011
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | Prior research studies on antibacterial agent and water disinfectants have revealed some problems associated with synthetic materials. Also, the search for new antimicrobial agents and natural disinfectants make different plant species and parts suitable for this purpose. In this research, various potentials of Nahar (Mesua ferrea) seed kernels (NSK) were studied. The proximate composition of both the seed kernels and hulls were determined using AOAC standard methods. The physicochemical properties of NSK oil extracts obtained from different extraction techniques (soxhlet and mechanical) were compared. The effects of solvent types, extraction time and temperature on NSK oil yield were also studied using soxhlet extraction method. The antimicrobial property of the extracts obtained from different extraction techniques (soxhlet and mechanical) and from different solvents (nhexane, ethanol, methanol, petroleum ether and chloroform) on two gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) and two gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) were also evaluated. Both the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of NSKO were also determined using broth dilution and agar diffusion techniques. Its disinfection property and kinetics were also evaluated using CFU/mL, pour plate method, 35ºC/48h, plate count agar of the heterotrophic plate count techniques. The dehulling of Nahar seed showed that the hull: kernel is 30:70, the proximate analysis of the seed kernel revealed that the seed kernel contained 3.55±0.50% moisture content, 76.0±2.0% crude oil, 1.50±0.20% crude protein, 1.25±0.20% ash content and 17.70±0.73% carbohydrate, while the hulls, contained 13.50±0.50% moisture content, 1.30±0.20% crude oil, 3.40±0.20% crude protein, 0.65±0.05% ash content and 81.15±0.24% carbohydrate. The analysis of the physicochemical properties of NSKO extracts obtained from different extraction techniques (soxhlet and mechanical) revealed that there were no significant differences (P>0.05) in the mean values of all the properties, showing that the extraction technique has no effect on the physico-chemical properties of the oils. The antimicrobial assay of the various samples of NSK oil obtained showed a remarkable inhibition zone and strong activity against all the tested bacteria with the Grampositive bacteria appeared to be more sensitive, more susceptible and less resistant. The MBC was found to be 2 mg/mL, while the MIC ranged from 1 mg/mL to 240 μg/mL. NSKO also showed remarkable disinfection potential, with total disinfection at 2 g/L and higher concentrations, while the kinetics studies at a concentration of 1 g/L suggested that NSKO fitted first-order model with a k value of -0.040. |
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| Item Description: | Abstract in English and Arabic. "A dissertation submitted in fulfilment of the requirement for the degree of Master of Science in Biotechnology Engineering."--On t.p. |
| Physical Description: | xvi, 117 leaves : ill. ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 93-107). |