Fundamental study of bioactive peptides on selected Alexandrium spp.at different growth phases /

The naturally occurring phenomenon of harmful algae blooming (HAB) at the water column brought detrimental effects to the economy as well as the environmental health of the water ecosystem. Most cases of HABs reported in Malaysia waters are dominated by dinoflagellates Alexandrium spp. with some of...

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Bibliographic Details
Main Author: Nurul Ashima Hamdan (Author)
Format: Thesis
Language:English
Published: Kuantan, Pahang : Kulliyyah of Science, International Islamic University Malaysia, 2020
Subjects:
Online Access:http://studentrepo.iium.edu.my/handle/123456789/10781
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040 |a UIAM  |b eng  |e rda 
041 |a eng 
043 |a a-my--- 
050 0 0 |a QP632.M37 
100 0 |a Nurul Ashima Hamdan,  |e author  |9 12009 
245 1 0 |a Fundamental study of bioactive peptides on selected Alexandrium spp.at different growth phases /  |c by Nurul Ashima bt Hamdan 
264 1 |a Kuantan, Pahang :  |b Kulliyyah of Science, International Islamic University Malaysia,  |c 2020 
300 |a xv, 90 leaves :  |b colour illustrations ;  |c 30cm. 
336 |2 rdacontent  |a text 
337 |2 rdamedia  |a unmediated 
337 |2 rdmedia  |a computer 
338 |2 rdacarrier  |a volume 
338 |2 rdacarrier  |a online resource 
347 |2 rdaft  |a text file  |b PDF 
500 |a Abstracts in English and Arabic. 
500 |a "A thesis submitted in fulfilment of the requirement for the degree of Master of Science (Biotechnology)." --On title page. 
502 |a Thesis (MSBTS)--International Islamic University Malaysia, 2020. 
504 |a Includes bibliographical references (leaves 69-84). 
520 |a The naturally occurring phenomenon of harmful algae blooming (HAB) at the water column brought detrimental effects to the economy as well as the environmental health of the water ecosystem. Most cases of HABs reported in Malaysia waters are dominated by dinoflagellates Alexandrium spp. with some of the species were reported to cause shellfish poisoning. In this study, two Alexandrium spp.; A. tamiyavanichii and A. leei with different toxicity levels were analyzed using proteomic approaches involving two-dimensional PAGE and HPLC analysis. The growth pattern of both species were identified and compared by using protein profiles at each growth phases. Protein expression reduced throughout the growth phases of A. tamiyavanichii but elevated during stationary phase of A. leei. A short duration of stationary phase suggests the continuous expression of growth proteins in A. leei. GNAT family acetyltransferase and lipases were successfully identified enzyme protein in A. tamiyavanichii and A. leei respectively with growth regulatory functions. The toxin profiles of both species exhibited higher level of toxin content in A. tamiyavanichii with 88 mol % of total toxins recorded as compared to 12 mol% in A. leei. The highest toxin content was recorded during exponential phase of A. tamiyavanichii with dominance of GTX4 and STX congeners. Further investigation on the toxicity effects of Alexandrium spp. was studied by using oysters where the shellfish are fed with toxic A. tamiyavanichii and non-toxic A. leei. LC-MS results of the two up-regulated spots in oysters fed with A. tamiyavanichii were identified as arginine kinase and transgelin. The up-regulation of the proteins was induced as responsive mechanism towards accumulation of the toxic species A. tamiyavanichii. These responses reflect the oyster stability during HAB outbreak. Fundamental studies of dinoflagellates from its molecular as well as byproduct analysis are useful to understand the biochemistry of the HAB species. The findings from this study can provide the basis knowledge on the biochemical properties of HAB species and the behavioral of affected organisms. 
650 0 |a Marine toxins  |x Toxicology  |9 12010 
650 0 |a Harmful Algal Bloom  |x physiology  |9 12011 
650 0 |a Environmental Exposure  |9 12012 
650 0 |a Alexandrium  |9 12013 
655 7 |a Theses, IIUM local 
690 |a Dissertations, Academic  |x Department of Biotechnology  |z IIUM  |9 10461 
700 0 |a Noraslinda Muhamad Bunnori,  |e degree supervisor  |9 12014 
700 0 |a Normawaty Mohammad Noor,  |e degree supervisor  |9 10301 
710 2 |a International Islamic University Malaysia.  |b Department of Biotechnology  |9 10464 
856 4 |u http://studentrepo.iium.edu.my/handle/123456789/10781 
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942 |2 lcc  |c THESIS 
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