Optimization of fat, oil, and grease (FOG) biodegradation using consortium of bacteria /
Fats, oils and grease (FOG) is a by-product produced originally by various food preparation activities. Removing FOG is critical because it is implicated in causing sewerage blockages leading to sewage system overflows (SSOs) and it can also deplete of oxygen levels in waterways. Recent studies favo...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur :
Kulliyyah of Engineering, International Islamic University Malaysia,
2019
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | Fats, oils and grease (FOG) is a by-product produced originally by various food preparation activities. Removing FOG is critical because it is implicated in causing sewerage blockages leading to sewage system overflows (SSOs) and it can also deplete of oxygen levels in waterways. Recent studies favoured biological methods over chemical and physical methods for it is more efficient, safe and eco-friendly approach in treating FOG in sewer system. This work aims to study the ability to degrade FOG by bacterial consortium isolated from palm oil mill effluent (POME). The consortium was prepared from bacteria strains which were capable of degrading FOG. Screening test on Tween 20 agar media was applied to identify the consortia capable of degrading FOG and determine best consortium. The results showed that the E3YZ consortium is best performing since it has largest growth and highest degradation of FOG. Optimization experiments were performed by using different levels of parameters to achieve the maximum removal efficiency of palm oil using the selected consortium. The parameters of pH (6, 7, 8), oil concentration (1, 3, 5% v/v), and bacterial inoculum concentration (2, 6, 10% v/v) were studied. The influence of these three parameters on the amount of oil degraded were investigated. Based on the results of 2 level factorial design, the most impact on the degraded oil was found in the oil concentration followed by the bacterial inoculum concentration then pH level. Oil concentration of 1% , 10% of bacterial inoculum concentration with pH 6 were obtained as the optimum conditions for oil degradation that led to the best degradation of FOG. At the optimum conditions, Consortium E3YZ, which comprises two strains, Micrococcus lylae strain DSM 20315 and Corynebacterium aurimucosum has shown the best result in the desired degradation of FOG. This consortium showed degradation up to 82.7% of FOG after 20 days of incubation. In conclusion, the study thus indicates that this bacterial consortium can be efficiently used for the biodegradation of FOG waste. |
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| Item Description: | Abstracts in English and Arabic. "A dissertation submitted in fulfilment of the requirement for the degree of Master of Science (Biotechnology Engineering)." --On title page. |
| Physical Description: | xiv, 84 leaves : colour illustrations ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 69-76). |