Potential biosurfactant-producing bacteria isolated from petroleum sludge /

Biosurfactants are amphiphilic compounds produced by microorganisms with almost similar properties as chemically synthesized surfactants. With amphiphilic properties, these compounds are widely applied as bioemulsifier and enhancer in the bioremediation process. This study aimed to screen potential...

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Bibliographic Details
Main Author: Nadhrah Annuar (Author)
Format: Thesis
Language:English
Published: Kuantan, Pahang : Kulliyyah of Science, International Islamic University Malaysia, 2021
Subjects:
Online Access:http://studentrepo.iium.edu.my/handle/123456789/11092
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040 |a UIAM  |b eng  |e rda 
041 |a eng 
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100 0 |a Nadhrah Annuar  |9 12016  |e author 
245 1 |a Potential biosurfactant-producing bacteria isolated from petroleum sludge /  |c by Nadhrah Annuar 
264 1 |a Kuantan, Pahang :   |b Kulliyyah of Science, International Islamic University Malaysia,   |c 2021 
300 |a xiv, 107 leaves :  |b colour illustrations ;  |c 30 cm. 
336 |a text  |2 rdacontent 
337 |a unmediated  |2 rdamedia 
337 |a computer  |2 rdamedia 
338 |a volume  |2 rdacarrier 
338 |a online resource  |2 rdacarrier 
347 |a text file  |b PDF  |2 rdaft 
500 |a Abstracts in English and Arabic. 
500 |a "A thesis submitted in fulfilment of the requirement for the degree of Master of Science (Biotechnology)." --On title page. 
502 |a Thesis (MSBTS)--International Islamic University Malaysia, 2021. 
504 |a Includes bibliographical references (leaves 86-96). 
520 |a Biosurfactants are amphiphilic compounds produced by microorganisms with almost similar properties as chemically synthesized surfactants. With amphiphilic properties, these compounds are widely applied as bioemulsifier and enhancer in the bioremediation process. This study aimed to screen potential biosurfactant producers from petroleum sludge. Bacteria were isolated from petroleum sludge samples and screened for biosurfactant activity by analysing Emulsification activity (E24) and Surface Tension of the culture supernatant. Selected isolates were subjected to 16S rRNA analysis while the isolate that showed the highest surface tension reduction was further subjected to the growth study. The effect of carbon and nitrogen source towards surface tension reduction was tested. Then, the biosurfactant produced was partially purified and characterized. Out of the 47 isolates, Pseudomonas sp. P15 exhibited among the highest emulsification index (67.41%) and biosurfactant activity by reducing the surface tension measurement as low as 18.92 mN/m. In the bacterial growth study, Pseudomonas sp. P15 exhibited the highest surface tension reduction when 2% of used engine oil (UE2) was used as the carbon source. The addition of nitrogen source, 0.5% of yeast extract did not affect the surface tension reduction but increased the bacteria growth and biosurfactant yield by 100-fold (43.37 g/L). After liquid-liquid extraction, the partially purified biosurfactant showed similar Rf value to the rhamnolipid standard in the thin layer chromatography (TLC) and effectively emulsified the hydrocarbon substrate. The finding indicated that Pseudomonas sp. P15 isolated from petroleum sludge has potential to produce biosurfactant that can be used in wide range of industrial application such as bioremediation of petroleum contaminated sites and enhance the oil recovery in the petroleum industry.  
655 |a Theses, IIUM local 
690 |a Dissertations, Academic  |x Department of Biotechnology  |z IIUM  |9 10461 
700 0 |a Mardiana Mohd Ashaari  |e degree supervisor  |9 12017 
700 0 |a Suhaila Mohd Omar  |e degree supervisor  |9 12018 
710 2 |a International Islamic University Malaysia.  |b Department of Biotechnology  |9 10464 
856 4 |u http://studentrepo.iium.edu.my/handle/123456789/11092 
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