The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan

NMDA-Rl subunit is important in synaptic plasticity and learning process. The aim of the present study was to determine the effect of HAB10R12 extract on the expression of N-Methyl-D-Aspartate Receptor-I subunit gene in mice brain after sub-acute exposure of HAB10R12. Thirty mice were randomly divid...

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Main Author:	Kasiman @ Kamisan, Norhidayah
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Pharmaceutical technology Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/101086/1/101086.PDF
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id	my-uitm-ir.101086
record_format	uketd_dc
spelling	my-uitm-ir.1010862024-09-28T23:32:53Z The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan 2008 Kasiman @ Kamisan, Norhidayah Pharmaceutical technology Pharmaceutical chemistry NMDA-Rl subunit is important in synaptic plasticity and learning process. The aim of the present study was to determine the effect of HAB10R12 extract on the expression of N-Methyl-D-Aspartate Receptor-I subunit gene in mice brain after sub-acute exposure of HAB10R12. Thirty mice were randomly divided into five groups which consist of normal saline, memantine, 5 mg/ml/kg, l O mg/ml/kg and 20 mg/ml/kg of HAB10R12 treatments. All the groups of mice were treated by intraperitoneal (IP) administration for 14 days. The mice were sacrificed at day I 5 and the brains were removed. RNA was extracted from the brains by using SY Total RNA Isolation System (Promega). QuantiFast SYBR Green RT-PCR Kit (Qiagen) was used to quantify the expression of NMDA-R1 subunit gene by using comparative threshold method that was recorded by Rotor-Gene 600 Series Software I. 7. The result showed that administration of 5mg/ml/kg HAB10R12 dose caused up-regulation of NMDA-R1 subunit gene in mice. In contrast, administration of 10mg/ml/kg HAB10R12 dose to mice cause down regulation of NMDA-R1 subunit gene. In conclusion, HAB10R12 might affect the expression ofNMDA-R1 subunit gene in mice brain after sub-acute exposure. 2008 Thesis https://ir.uitm.edu.my/id/eprint/101086/ https://ir.uitm.edu.my/id/eprint/101086/1/101086.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Pharmaceutical technology Pharmaceutical chemistry
spellingShingle	Pharmaceutical technology Pharmaceutical chemistry Kasiman @ Kamisan, Norhidayah The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan
description	NMDA-Rl subunit is important in synaptic plasticity and learning process. The aim of the present study was to determine the effect of HAB10R12 extract on the expression of N-Methyl-D-Aspartate Receptor-I subunit gene in mice brain after sub-acute exposure of HAB10R12. Thirty mice were randomly divided into five groups which consist of normal saline, memantine, 5 mg/ml/kg, l O mg/ml/kg and 20 mg/ml/kg of HAB10R12 treatments. All the groups of mice were treated by intraperitoneal (IP) administration for 14 days. The mice were sacrificed at day I 5 and the brains were removed. RNA was extracted from the brains by using SY Total RNA Isolation System (Promega). QuantiFast SYBR Green RT-PCR Kit (Qiagen) was used to quantify the expression of NMDA-R1 subunit gene by using comparative threshold method that was recorded by Rotor-Gene 600 Series Software I. 7. The result showed that administration of 5mg/ml/kg HAB10R12 dose caused up-regulation of NMDA-R1 subunit gene in mice. In contrast, administration of 10mg/ml/kg HAB10R12 dose to mice cause down regulation of NMDA-R1 subunit gene. In conclusion, HAB10R12 might affect the expression ofNMDA-R1 subunit gene in mice brain after sub-acute exposure.
format	Thesis
qualification_level	Bachelor degree
author	Kasiman @ Kamisan, Norhidayah
author_facet	Kasiman @ Kamisan, Norhidayah
author_sort	Kasiman @ Kamisan, Norhidayah
title	The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan
title_short	The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan
title_full	The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan
title_fullStr	The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan
title_full_unstemmed	The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan
title_sort	effects of sub-acute exposure of hab10r12 ethanolic extract on the expression of n-methyl-d-aspartate receptor-i subunit gene in mice brain / norhidayah kasiman @ kamisan
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/101086/1/101086.PDF
_version_	1811769128480931840

The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan

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