Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor

Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor

Morinda citrifolia is said to have countless biological activity. Damnacanthal and nordamnacanthal listed under anthraquinones group which were identified to be the major compounds in roots of Morinda citrifolia. Isolation and purification of both compounds were the interest in this research. 50:50...

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Main Author: Mohd Noor, Izdihar
Format: Thesis
Language:English
Published: 2010
Online Access:https://ir.uitm.edu.my/id/eprint/102587/1/102587.pdf
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spelling my-uitm-ir.1025872024-09-23T01:24:34Z Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor 2010 Mohd Noor, Izdihar Morinda citrifolia is said to have countless biological activity. Damnacanthal and nordamnacanthal listed under anthraquinones group which were identified to be the major compounds in roots of Morinda citrifolia. Isolation and purification of both compounds were the interest in this research. 50:50 dichloromethane:hexane solvent was used as the mobile phase in column chromatography packed with acid-washed 1 I Q silica to isolate and purify both damnacanthal and nordamnacanthal. H and C NMR were carried out to elucidate on the structure of both compounds collected. Besides that, the investigation of other biological activity of both damnacanthal and nordamnacanthal were conducted. Solvent toxicity, IC50 of hydrogen peroxide toxicity, neurotoxicity and neuroprotection assays were done on SH-SY5Y cell by conducting cell viability test of MTT. The result of neurotoxicity and neuroprotective effect of both compounds were determined by the percentage of cell viability of the SH-SY5Y cell. 2010 Thesis https://ir.uitm.edu.my/id/eprint/102587/ https://ir.uitm.edu.my/id/eprint/102587/1/102587.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
description Morinda citrifolia is said to have countless biological activity. Damnacanthal and nordamnacanthal listed under anthraquinones group which were identified to be the major compounds in roots of Morinda citrifolia. Isolation and purification of both compounds were the interest in this research. 50:50 dichloromethane:hexane solvent was used as the mobile phase in column chromatography packed with acid-washed 1 I Q silica to isolate and purify both damnacanthal and nordamnacanthal. H and C NMR were carried out to elucidate on the structure of both compounds collected. Besides that, the investigation of other biological activity of both damnacanthal and nordamnacanthal were conducted. Solvent toxicity, IC50 of hydrogen peroxide toxicity, neurotoxicity and neuroprotection assays were done on SH-SY5Y cell by conducting cell viability test of MTT. The result of neurotoxicity and neuroprotective effect of both compounds were determined by the percentage of cell viability of the SH-SY5Y cell.
format Thesis
qualification_level Bachelor degree
author Mohd Noor, Izdihar
spellingShingle Mohd Noor, Izdihar
Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor
author_facet Mohd Noor, Izdihar
author_sort Mohd Noor, Izdihar
title Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor
title_short Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor
title_full Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor
title_fullStr Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor
title_full_unstemmed Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor
title_sort isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / izdihar mohd noor
granting_institution Universiti Teknologi MARA
granting_department Faculty of Applied Sciences
publishDate 2010
url https://ir.uitm.edu.my/id/eprint/102587/1/102587.pdf
_version_ 1811769227355357184

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