Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat

Thromboxane synthase gene (TBXAS 1), converts prostaglandin H2 into thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. Thromboxane A2 has been implicated in modulating cell cytotoxicity tumor growth and metastasis. The aim of this study is to develop and validate a simple...

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Main Author:	Rahmat, Roihanah
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical technology Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/105125/1/105125.PDF
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id	my-uitm-ir.105125
record_format	uketd_dc
spelling	my-uitm-ir.1051252024-10-24T01:45:09Z Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat 2009 Rahmat, Roihanah Pharmaceutical technology Pharmaceutical chemistry Thromboxane synthase gene (TBXAS 1), converts prostaglandin H2 into thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. Thromboxane A2 has been implicated in modulating cell cytotoxicity tumor growth and metastasis. The aim of this study is to develop and validate a simple PCR based method to detect the allelic variance of thromboxane synthase gene. The primers were designed according to the gene sequence. Polymerase Chain Reaction using first and second set of primers was done to amplify the sequence of TBXASl. Besides, DNA collection methods arise from blood samples and buccal samples also successfully done in detection of wild-type and mutated alleles respectively by using common reverse primer plus wild-type forward primer and mutant forward primer. Based on the result of this study, it can be concluded that the amplification process is successful based on the correct band size obtained. From the PCR product, there were some bands that shows heterozygous and homozygous for allelic variance in TBXASl gene. For further confirmation, the PCR product that was obtained in this study would be sent for sequencing and compared with published sequences. 2009 Thesis https://ir.uitm.edu.my/id/eprint/105125/ https://ir.uitm.edu.my/id/eprint/105125/1/105125.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Teh, Lay Kek
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Teh, Lay Kek
topic	Pharmaceutical technology Pharmaceutical chemistry
spellingShingle	Pharmaceutical technology Pharmaceutical chemistry Rahmat, Roihanah Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat
description	Thromboxane synthase gene (TBXAS 1), converts prostaglandin H2 into thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. Thromboxane A2 has been implicated in modulating cell cytotoxicity tumor growth and metastasis. The aim of this study is to develop and validate a simple PCR based method to detect the allelic variance of thromboxane synthase gene. The primers were designed according to the gene sequence. Polymerase Chain Reaction using first and second set of primers was done to amplify the sequence of TBXASl. Besides, DNA collection methods arise from blood samples and buccal samples also successfully done in detection of wild-type and mutated alleles respectively by using common reverse primer plus wild-type forward primer and mutant forward primer. Based on the result of this study, it can be concluded that the amplification process is successful based on the correct band size obtained. From the PCR product, there were some bands that shows heterozygous and homozygous for allelic variance in TBXASl gene. For further confirmation, the PCR product that was obtained in this study would be sent for sequencing and compared with published sequences.
format	Thesis
qualification_level	Bachelor degree
author	Rahmat, Roihanah
author_facet	Rahmat, Roihanah
author_sort	Rahmat, Roihanah
title	Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat
title_short	Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat
title_full	Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat
title_fullStr	Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat
title_full_unstemmed	Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat
title_sort	development of a pcr method for detection of thromboxane synthase gene / roihanah rahmat
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/105125/1/105125.PDF
_version_	1818588137054535680

Development of a PCR method for detection of thromboxane synthase gene / Roihanah Rahmat

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