Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim

Long range PCR method for uniform amplification of specific lambda DNA sequence is described. The careful design of all 6 pairs of primers and optimum temperature cycling profile produce good result for long range PCR. Three pairs of 15kb and three pairs of 20kb primers were design using oligo explo...

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Main Author:	Mohamad Kassim, Muhamad Saifuzzaman
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical industry Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/105247/1/105247.pdf
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id	my-uitm-ir.105247
record_format	uketd_dc
spelling	my-uitm-ir.1052472024-12-05T09:00:06Z Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim 2009 Mohamad Kassim, Muhamad Saifuzzaman Pharmaceutical industry Pharmaceutical chemistry Long range PCR method for uniform amplification of specific lambda DNA sequence is described. The careful design of all 6 pairs of primers and optimum temperature cycling profile produce good result for long range PCR. Three pairs of 15kb and three pairs of 20kb primers were design using oligo explorer 1.2. Four out of six (All three 15kb PCR and one 20kb PCR) produce results. Two variables that affect the efficiencies of long range PCR were studied which consists of the percentage of DMSO added and the concentration of MgCb used in the PCR. A combination of 5%, 7%, and I 0% DMSO were added to study the improvement of the efficiencies of long range PCR amplification that theoretically should increase the ratio of full-length products to shortened products. Results were somehow unobtainable and optimum percentage of DMSO for long range PCR is not known. The differing MgCb concentrations were added and the result was also unobtainable and optimum concentration of free Mg2+ in the PCR mix is not known. 2009 Thesis https://ir.uitm.edu.my/id/eprint/105247/ https://ir.uitm.edu.my/id/eprint/105247/1/105247.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Mohd Yusoff, Rosmadi
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mohd Yusoff, Rosmadi
topic	Pharmaceutical industry Pharmaceutical chemistry
spellingShingle	Pharmaceutical industry Pharmaceutical chemistry Mohamad Kassim, Muhamad Saifuzzaman Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim
description	Long range PCR method for uniform amplification of specific lambda DNA sequence is described. The careful design of all 6 pairs of primers and optimum temperature cycling profile produce good result for long range PCR. Three pairs of 15kb and three pairs of 20kb primers were design using oligo explorer 1.2. Four out of six (All three 15kb PCR and one 20kb PCR) produce results. Two variables that affect the efficiencies of long range PCR were studied which consists of the percentage of DMSO added and the concentration of MgCb used in the PCR. A combination of 5%, 7%, and I 0% DMSO were added to study the improvement of the efficiencies of long range PCR amplification that theoretically should increase the ratio of full-length products to shortened products. Results were somehow unobtainable and optimum percentage of DMSO for long range PCR is not known. The differing MgCb concentrations were added and the result was also unobtainable and optimum concentration of free Mg2+ in the PCR mix is not known.
format	Thesis
qualification_level	Bachelor degree
author	Mohamad Kassim, Muhamad Saifuzzaman
author_facet	Mohamad Kassim, Muhamad Saifuzzaman
author_sort	Mohamad Kassim, Muhamad Saifuzzaman
title	Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim
title_short	Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim
title_full	Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim
title_fullStr	Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim
title_full_unstemmed	Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim
title_sort	investigation of the factors that influence long range pcr / muhamad saifuzzaman mohamad kassim
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/105247/1/105247.pdf
_version_	1818588143535783936

Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim

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