Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim
Long range PCR method for uniform amplification of specific lambda DNA sequence is described. The careful design of all 6 pairs of primers and optimum temperature cycling profile produce good result for long range PCR. Three pairs of 15kb and three pairs of 20kb primers were design using oligo explo...
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Format: | Thesis |
Language: | English |
Published: |
2009
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Online Access: | https://ir.uitm.edu.my/id/eprint/105247/1/105247.pdf |
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