Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof

Malaysian traditional herbs had been observed to be more popular among Malaysian populations. People belief that traditional herbs is more curative than modem medicine without notice that herbs also contains constituents that can undergo some effect on metabolizing enzyme inside human body. This exp...

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Main Author:	Yusof, Nurulhusna Syuhaidah
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical industry Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/105524/1/105524.PDF
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id	my-uitm-ir.105524
record_format	uketd_dc
spelling	my-uitm-ir.1055242024-12-05T08:48:57Z Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof 2009 Yusof, Nurulhusna Syuhaidah Pharmaceutical industry Pharmaceutical chemistry Malaysian traditional herbs had been observed to be more popular among Malaysian populations. People belief that traditional herbs is more curative than modem medicine without notice that herbs also contains constituents that can undergo some effect on metabolizing enzyme inside human body. This experiment will evaluate the interactions of 56 species of Malaysian traditional herbs with CYP1A2 enzyme. In human liver, CYP 1 A2 enzyme consists of about 10-15% of the total CYP content of human liver. This assay had used Vivid® CYP450 Screening Kits which designed to assess metabolism and inhibition of the predominant human P450 isozymes involved in hepatic metabolism. By using this kits, interactions of 56 species of Malaysian traditional herbs, n-naphthoflavone (positive control) and dimethyl sulfoxide, DMSO (solvent control) at CYP 1 A2 enzyme were analyzed through intensity of fluorescent. Based on this study, it is showed that DMSO (solvent control) has higher fluorescent intensity. This means that it has no inhibition at CYP1A2 enzyme. A-naphthoflavone (positive control) had showed inhibition at CYP1A2 enzyme as low fluorescent intensity was observed. Meanwhile, among 56 species of these Malaysian traditional herbs, 18 samples of these herbs, including misai kucing, pegaga, pinang and tongkat ali had showed positive inhibition at CYP l A2 enzyme. 2009 Thesis https://ir.uitm.edu.my/id/eprint/105524/ https://ir.uitm.edu.my/id/eprint/105524/1/105524.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Choo, Chee Yan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Choo, Chee Yan
topic	Pharmaceutical industry Pharmaceutical chemistry
spellingShingle	Pharmaceutical industry Pharmaceutical chemistry Yusof, Nurulhusna Syuhaidah Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof
description	Malaysian traditional herbs had been observed to be more popular among Malaysian populations. People belief that traditional herbs is more curative than modem medicine without notice that herbs also contains constituents that can undergo some effect on metabolizing enzyme inside human body. This experiment will evaluate the interactions of 56 species of Malaysian traditional herbs with CYP1A2 enzyme. In human liver, CYP 1 A2 enzyme consists of about 10-15% of the total CYP content of human liver. This assay had used Vivid® CYP450 Screening Kits which designed to assess metabolism and inhibition of the predominant human P450 isozymes involved in hepatic metabolism. By using this kits, interactions of 56 species of Malaysian traditional herbs, n-naphthoflavone (positive control) and dimethyl sulfoxide, DMSO (solvent control) at CYP 1 A2 enzyme were analyzed through intensity of fluorescent. Based on this study, it is showed that DMSO (solvent control) has higher fluorescent intensity. This means that it has no inhibition at CYP1A2 enzyme. A-naphthoflavone (positive control) had showed inhibition at CYP1A2 enzyme as low fluorescent intensity was observed. Meanwhile, among 56 species of these Malaysian traditional herbs, 18 samples of these herbs, including misai kucing, pegaga, pinang and tongkat ali had showed positive inhibition at CYP l A2 enzyme.
format	Thesis
qualification_level	Bachelor degree
author	Yusof, Nurulhusna Syuhaidah
author_facet	Yusof, Nurulhusna Syuhaidah
author_sort	Yusof, Nurulhusna Syuhaidah
title	Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof
title_short	Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof
title_full	Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof
title_fullStr	Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof
title_full_unstemmed	Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof
title_sort	malaysian traditional herb: cyp - herb interaction on cyp1a2 enzyme / nurulhusna syuhaidah yusof
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/105524/1/105524.PDF
_version_	1818588150861135872

Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof

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