Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman

DNA ladder is an important tool in Gel Electrophoresis to determine the size of DNA fragment of (Polymerase Chain Reaction) PCR products. It has two types which is low molecular weight and high molecular weight DNA ladder. The objective of this research is to synthesize low molecular weight DNA ladd...

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Main Author:	Kamarulzaman, Nurul Farhana
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical industry Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/105840/1/105840.PDF
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id	my-uitm-ir.105840
record_format	uketd_dc
spelling	my-uitm-ir.1058402024-12-05T08:12:27Z Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman 2009 Kamarulzaman, Nurul Farhana Pharmaceutical industry Pharmaceutical chemistry DNA ladder is an important tool in Gel Electrophoresis to determine the size of DNA fragment of (Polymerase Chain Reaction) PCR products. It has two types which is low molecular weight and high molecular weight DNA ladder. The objective of this research is to synthesize low molecular weight DNA ladder. It is also known as 100bp DNA ladder. There are several methods to synthesize low molecular weight DNA ladder. In this research, the method used is by PCR amplification of lambda phage DNA. It is done by designing PCR primer for each length of I OObp ladder. Then,PCR process is done and continued by running gel electrophoresis. After that, the gel is screened through UV transilluminator. As a result, there are several successful PCR product which are 200bp, 300bp, 400bp,600bp, 700bp, 800bp and 1000bp. The remaining shows failure of PCR process although the procedures are repeated for several times. The reason of failure is maybe due to contamination of reagents used. Although there are failure for some of the PCR amplification, but the method to synthesize of low molecular weight DNA ladder using PCR amplification is really useful to reduce laboratory cost compared buying it from the commercial DNA ladder. 2009 Thesis https://ir.uitm.edu.my/id/eprint/105840/ https://ir.uitm.edu.my/id/eprint/105840/1/105840.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Md Yussof, Rosmadi
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Md Yussof, Rosmadi
topic	Pharmaceutical industry Pharmaceutical chemistry
spellingShingle	Pharmaceutical industry Pharmaceutical chemistry Kamarulzaman, Nurul Farhana Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman
description	DNA ladder is an important tool in Gel Electrophoresis to determine the size of DNA fragment of (Polymerase Chain Reaction) PCR products. It has two types which is low molecular weight and high molecular weight DNA ladder. The objective of this research is to synthesize low molecular weight DNA ladder. It is also known as 100bp DNA ladder. There are several methods to synthesize low molecular weight DNA ladder. In this research, the method used is by PCR amplification of lambda phage DNA. It is done by designing PCR primer for each length of I OObp ladder. Then,PCR process is done and continued by running gel electrophoresis. After that, the gel is screened through UV transilluminator. As a result, there are several successful PCR product which are 200bp, 300bp, 400bp,600bp, 700bp, 800bp and 1000bp. The remaining shows failure of PCR process although the procedures are repeated for several times. The reason of failure is maybe due to contamination of reagents used. Although there are failure for some of the PCR amplification, but the method to synthesize of low molecular weight DNA ladder using PCR amplification is really useful to reduce laboratory cost compared buying it from the commercial DNA ladder.
format	Thesis
qualification_level	Bachelor degree
author	Kamarulzaman, Nurul Farhana
author_facet	Kamarulzaman, Nurul Farhana
author_sort	Kamarulzaman, Nurul Farhana
title	Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman
title_short	Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman
title_full	Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman
title_fullStr	Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman
title_full_unstemmed	Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman
title_sort	synthesis of low molecular weight dna ladder / nurul farhana kamarulzaman
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/105840/1/105840.PDF
_version_	1818588158123573248

Synthesis of low molecular weight DNA ladder / Nurul Farhana Kamarulzaman

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