Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa

Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa

The World Health Organization (WHO) reported in 2007 that 7.9 million deaths worldwide (or 13% of total deaths) due to cancer, and the most common cancer in the world are lung, breast and colorectal cancer. 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay was used in the in...

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Main Author: Mustaffa, Mohd Faiz
Format: Thesis
Language:English
Published: 2009
Subjects:
Pharmaceutical industry
Online Access:https://ir.uitm.edu.my/id/eprint/107035/1/107035.PDF
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spelling my-uitm-ir.1070352024-12-10T23:12:48Z Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa 2009 Mustaffa, Mohd Faiz Pharmaceutical industry The World Health Organization (WHO) reported in 2007 that 7.9 million deaths worldwide (or 13% of total deaths) due to cancer, and the most common cancer in the world are lung, breast and colorectal cancer. 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay was used in the in vitro anticancer activity of Muntingia Calabura methanol extracts studies against MCF-7, HL60 and A549 cancer cell lines. M Calabura fresh leaves were air-dried approximately 2 weeks then soaked in methanol prior evaporated to dryness. The methanol crude partitioned sequentially in hexane, chloroform and methanol itself and subjected to anticancer activity. Methanol partition showed potent anticancer activity compare with others partition and methanol crude against MCF-7 (8.5 µg/mL), HL60 (3.2 µg/mL) A549 cancer cell (11 µg/mL). The potent activity of methanol partition towards both cancer cell lines was prior subjected to fractionation by using vacuum liquid chromatography (VLC). The fractions of methanol partition showed less potent against tested cancer cell line as compared to methanol partition itself exclude F5 and F6 tested on MCF-7 cancer cell line (5.5 and 7 µg/mL). Interestingly, all fractions failed to exert cytotoxic activities on cancer cell line especially on MCF-7, possessed cytotoxic activities on normal cell line, 3T3, which indicates the unsafe used of the fractions as chemotherapy. In conclusion, the extracts of M Calabura possess potential anticancer activity that requires further thorough studies. 2009 Thesis https://ir.uitm.edu.my/id/eprint/107035/ https://ir.uitm.edu.my/id/eprint/107035/1/107035.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Zakaria, Zainul Aminuddin
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Zakaria, Zainul Aminuddin
topic Pharmaceutical industry
spellingShingle Pharmaceutical industry
Mustaffa, Mohd Faiz
Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa
description The World Health Organization (WHO) reported in 2007 that 7.9 million deaths worldwide (or 13% of total deaths) due to cancer, and the most common cancer in the world are lung, breast and colorectal cancer. 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay was used in the in vitro anticancer activity of Muntingia Calabura methanol extracts studies against MCF-7, HL60 and A549 cancer cell lines. M Calabura fresh leaves were air-dried approximately 2 weeks then soaked in methanol prior evaporated to dryness. The methanol crude partitioned sequentially in hexane, chloroform and methanol itself and subjected to anticancer activity. Methanol partition showed potent anticancer activity compare with others partition and methanol crude against MCF-7 (8.5 µg/mL), HL60 (3.2 µg/mL) A549 cancer cell (11 µg/mL). The potent activity of methanol partition towards both cancer cell lines was prior subjected to fractionation by using vacuum liquid chromatography (VLC). The fractions of methanol partition showed less potent against tested cancer cell line as compared to methanol partition itself exclude F5 and F6 tested on MCF-7 cancer cell line (5.5 and 7 µg/mL). Interestingly, all fractions failed to exert cytotoxic activities on cancer cell line especially on MCF-7, possessed cytotoxic activities on normal cell line, 3T3, which indicates the unsafe used of the fractions as chemotherapy. In conclusion, the extracts of M Calabura possess potential anticancer activity that requires further thorough studies.
format Thesis
qualification_level Bachelor degree
author Mustaffa, Mohd Faiz
author_facet Mustaffa, Mohd Faiz
author_sort Mustaffa, Mohd Faiz
title Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa
title_short Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa
title_full Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa
title_fullStr Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa
title_full_unstemmed Antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / Mohd Faiz Mustaffa
title_sort antiproliferative activity of various fractions from methanol extract of the leaves of muntingia calabura / mohd faiz mustaffa
granting_institution Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department Faculty of Pharmacy
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/107035/1/107035.PDF
_version_ 1818588202831708160

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