Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib

During canning and juice processing of pineapple, pineapple peel usually discharged. Discharged of pineapple peel during these productions will produce waste and lead to serious environmental pollution. In industrial practices, pineapple waste is either used as animal feed or disposed to the soil as...

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Main Author:	Ab. Mutalib, Siti Roha
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Indexes (General)
Online Access:	https://ir.uitm.edu.my/id/eprint/16325/6/16325.pdf
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id	my-uitm-ir.16325
record_format	uketd_dc
spelling	my-uitm-ir.163252022-12-16T02:42:09Z Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib 2015-01 Ab. Mutalib, Siti Roha Indexes (General) During canning and juice processing of pineapple, pineapple peel usually discharged. Discharged of pineapple peel during these productions will produce waste and lead to serious environmental pollution. In industrial practices, pineapple waste is either used as animal feed or disposed to the soil as waste. Pineapple peel contain valuable natural enzyme which is bromelain. Bromelain is enzyme which believed to have many benefits and very promising to the development of food and pharmaceutical industries. The purpose of this study is to isolate leucine from beef by using purified bromelain from pineapple peel extract. Purified bromelain powder from pineapple peel was produced through purification process. These include extraction from pineapple peel using purified water as a medium extraction, purification by cation exchange chromatography, desalting using continuous diafiltrator and followed by freeze drying. Each step was found to produce different effect on bromelain activity, protein content, specific enzyme activity and purification level. It was found that bromelain extracted from 100g of pineapple peel could yield 1.0g of bromelain powder. The amino acid composition in 14 beef cuts was also determined in this study. The flank cut was found to contain significantly higher amount of leucine amongst the beef cuts. The enzymatic hydrolysis was done with bromelain enzyme as a substrate to produce beef protein hydrolysate with higher content of leucine. The optimisation condition for the isolation of leucine was found with bromelain concentration of 1.380/0, hydrolysis temperature of 42.5°C and hydrolysis time of 31.59 hours. The isolation of leucine was done by cation exchange chromatography and followed by freeze drying to obtain leucine powder. The leucine produce are as crystalline solid form, clean and white deposit. This leucine powder was used to determine the secretion of insulin in plasma of male Sprague-Dawley rats. It was found that the mean plasma insulin concentration value was achieved maximum at 90 minutes and 180 minutes for intramuscular injection and oral, respectively after leucine administered. The percentage of insulin increment for both intramuscular injection and oral administration of leucine are 80.40% and 79.02%, respectively. 2015-01 Thesis https://ir.uitm.edu.my/id/eprint/16325/ https://ir.uitm.edu.my/id/eprint/16325/6/16325.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Samicho, Zainal
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Samicho, Zainal
topic	Indexes (General)
spellingShingle	Indexes (General) Ab. Mutalib, Siti Roha Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib
description	During canning and juice processing of pineapple, pineapple peel usually discharged. Discharged of pineapple peel during these productions will produce waste and lead to serious environmental pollution. In industrial practices, pineapple waste is either used as animal feed or disposed to the soil as waste. Pineapple peel contain valuable natural enzyme which is bromelain. Bromelain is enzyme which believed to have many benefits and very promising to the development of food and pharmaceutical industries. The purpose of this study is to isolate leucine from beef by using purified bromelain from pineapple peel extract. Purified bromelain powder from pineapple peel was produced through purification process. These include extraction from pineapple peel using purified water as a medium extraction, purification by cation exchange chromatography, desalting using continuous diafiltrator and followed by freeze drying. Each step was found to produce different effect on bromelain activity, protein content, specific enzyme activity and purification level. It was found that bromelain extracted from 100g of pineapple peel could yield 1.0g of bromelain powder. The amino acid composition in 14 beef cuts was also determined in this study. The flank cut was found to contain significantly higher amount of leucine amongst the beef cuts. The enzymatic hydrolysis was done with bromelain enzyme as a substrate to produce beef protein hydrolysate with higher content of leucine. The optimisation condition for the isolation of leucine was found with bromelain concentration of 1.380/0, hydrolysis temperature of 42.5°C and hydrolysis time of 31.59 hours. The isolation of leucine was done by cation exchange chromatography and followed by freeze drying to obtain leucine powder. The leucine produce are as crystalline solid form, clean and white deposit. This leucine powder was used to determine the secretion of insulin in plasma of male Sprague-Dawley rats. It was found that the mean plasma insulin concentration value was achieved maximum at 90 minutes and 180 minutes for intramuscular injection and oral, respectively after leucine administered. The percentage of insulin increment for both intramuscular injection and oral administration of leucine are 80.40% and 79.02%, respectively.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ab. Mutalib, Siti Roha
author_facet	Ab. Mutalib, Siti Roha
author_sort	Ab. Mutalib, Siti Roha
title	Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib
title_short	Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib
title_full	Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib
title_fullStr	Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib
title_full_unstemmed	Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib
title_sort	physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / siti roha ab. mutalib
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/16325/6/16325.pdf
_version_	1783733504503185408

Physico-chemical properties of pineapple peel extract and its application to isolate leucine from beef / Siti Roha Ab. Mutalib

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