Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan

In the current era of market globalization, people in the world could not evade from imported food products. The demand for imported food products such as chocolates, biscuits and sweets are projected to escalate steadily over the next decade as a result of increasing consumption. Unfortunately, mos...

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Main Author:	Hassan, Khairunnisa
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/17906/2/TM_KHAIRUNNISA%20HASSAN%20AS%2016_5.pdf
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id	my-uitm-ir.17906
record_format	uketd_dc
spelling	my-uitm-ir.179062022-03-08T07:18:08Z Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan 2016 Hassan, Khairunnisa In the current era of market globalization, people in the world could not evade from imported food products. The demand for imported food products such as chocolates, biscuits and sweets are projected to escalate steadily over the next decade as a result of increasing consumption. Unfortunately, most of the imported food products do not have Halal Logo or with doubted Halal Logo. The demand for Halal food and other Islamic consumer goods is increasing. This study will be beneficial to provide new information of Halal products and easier for Muslim to choose the permissible products according to Syariah Law. The main objective of this study was to determine of suspected Non Halal processed food products by using porcine mitochondrial 12S rDNA and porcine leptin gene. A total of 66 samples of suspected Non Halal food products were screened for porcine mitochondrial 12S rDNA gene and porcine leptin gene primer pairs from the genomic DNA. The PCR products were separated on 2% agarose gel and visualized under UV light. Thirty seven samples were positive with mitochondrial 12S rDNA gene whilst 59 samples were positive with leptin gene. From these, 33 were positive with both primers. These results indicate that the samples of processed food products contained porcine derivatives. From the detection of the DNA products by using the two set of primers, leptin gene was concluded to be more specific than the mitochondrial 12S rDNA gene. Some of the PCR products of processed food products of mitochondrial 12S rDNA gene and leptin gene were sent to Genomic Bioscience & Technology Company for DNA sequencing. Then, the sequences of the DNA were used for sequence alignment in order to get a probe specific to Halal food. Two probes were obtained, one with 24 mers and 13 mers, respectively. Mitochondrial 12S rDNA gene was chosen to make a probe because it has more quality DNA for a probe compared to leptin gene. In addition, findings from this research also provide new information in the detection of pork in foods products for Halal authentication. 2016 Thesis https://ir.uitm.edu.my/id/eprint/17906/ https://ir.uitm.edu.my/id/eprint/17906/2/TM_KHAIRUNNISA%20HASSAN%20AS%2016_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	In the current era of market globalization, people in the world could not evade from imported food products. The demand for imported food products such as chocolates, biscuits and sweets are projected to escalate steadily over the next decade as a result of increasing consumption. Unfortunately, most of the imported food products do not have Halal Logo or with doubted Halal Logo. The demand for Halal food and other Islamic consumer goods is increasing. This study will be beneficial to provide new information of Halal products and easier for Muslim to choose the permissible products according to Syariah Law. The main objective of this study was to determine of suspected Non Halal processed food products by using porcine mitochondrial 12S rDNA and porcine leptin gene. A total of 66 samples of suspected Non Halal food products were screened for porcine mitochondrial 12S rDNA gene and porcine leptin gene primer pairs from the genomic DNA. The PCR products were separated on 2% agarose gel and visualized under UV light. Thirty seven samples were positive with mitochondrial 12S rDNA gene whilst 59 samples were positive with leptin gene. From these, 33 were positive with both primers. These results indicate that the samples of processed food products contained porcine derivatives. From the detection of the DNA products by using the two set of primers, leptin gene was concluded to be more specific than the mitochondrial 12S rDNA gene. Some of the PCR products of processed food products of mitochondrial 12S rDNA gene and leptin gene were sent to Genomic Bioscience & Technology Company for DNA sequencing. Then, the sequences of the DNA were used for sequence alignment in order to get a probe specific to Halal food. Two probes were obtained, one with 24 mers and 13 mers, respectively. Mitochondrial 12S rDNA gene was chosen to make a probe because it has more quality DNA for a probe compared to leptin gene. In addition, findings from this research also provide new information in the detection of pork in foods products for Halal authentication.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Hassan, Khairunnisa
spellingShingle	Hassan, Khairunnisa Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
author_facet	Hassan, Khairunnisa
author_sort	Hassan, Khairunnisa
title	Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_short	Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_full	Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_fullStr	Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_full_unstemmed	Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_sort	determination of suspected non halal food products by using porcine mitochondrial 12s rdna and porcine leptin gene / khairunnisa hassan
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/17906/2/TM_KHAIRUNNISA%20HASSAN%20AS%2016_5.pdf
_version_	1783733622073720832

Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan

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