In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi

A study was conducted to determine the effects of different plant regulators (PGR) towards in vitro regeneration and callus induction of new Malaysian rice variety of Oryza sativa L. var. MRIA 1. Intemode explants of 4-week old of aseptic seedlings were selected for in vitro regeneration and a total...

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Main Author:	Mohamad Fauzi, Nor Yasmin
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Plant anatomy Plant physiology Plant ecology
Online Access:	https://ir.uitm.edu.my/id/eprint/27186/1/TM_NOR%20YASMIN%20MOHAMAD%20FAUZI%20AS%2016_5.pdf
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spelling	my-uitm-ir.271862022-11-04T00:16:08Z In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi 2016 Mohamad Fauzi, Nor Yasmin Plant anatomy Plant physiology Plant ecology A study was conducted to determine the effects of different plant regulators (PGR) towards in vitro regeneration and callus induction of new Malaysian rice variety of Oryza sativa L. var. MRIA 1. Intemode explants of 4-week old of aseptic seedlings were selected for in vitro regeneration and a total of 16 types of different combinations of NAA, IBA, BAP, and Kn were utilised. The most suitable plant growth regulators (PGR) for regeneration of Oryza sativa L. var. MRIA 1 was obtained from MS (Murashige and Skoog) media supplemented with 1.0 mg/L NAA and 0.5 mg/L Kn with an average of 8.90 ± 1.79 number of shoots per explant, 114.00 ± 18.90 number of roots per explant, 10.70 ± 2.62 cm length of shoots per explant and 8.70 ± 1.64 number of leaves per explant. The effect of combination of different plant growth regulators (PGR) supplemented in culture media on dry weight of regenerated Oryza sativa L. var. MRIA 1 were determined. Highest fresh weight (4.35±1.57 g) and dry weight (0.44±0.11 g per explant) recorded on the MS media supplemented with 1.0 mg/L NAA and 0.5 mg/L Kn. The callus inductions were assessed from three different explants including root, internode, and leaf base. The findings showed that the addition of 1.0 mg/L BAP into MS media was the most suitable for callus induction from root explant since it yield the highest fresh weight (1.039 ± 0.935 g per explant) and dry weight (0.093 ± 0.065 g per explant). The findings provided and optimum plant growth regulators (PGR) concentration for the tissue culture sysytem of Oryza sativa L. var. MRIA 1. Acclimatization of regenerated Oryza sativa L. var. MRIA 1 is recommended in order to serve a complete in vitro regeneration protocol of Oryza sativa L. var. MRIA 1. 2016 Thesis https://ir.uitm.edu.my/id/eprint/27186/ https://ir.uitm.edu.my/id/eprint/27186/1/TM_NOR%20YASMIN%20MOHAMAD%20FAUZI%20AS%2016_5.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences Jaafar Sidik, Norrizah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Jaafar Sidik, Norrizah
topic	Plant anatomy Plant physiology Plant ecology
spellingShingle	Plant anatomy Plant physiology Plant ecology Mohamad Fauzi, Nor Yasmin In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
description	A study was conducted to determine the effects of different plant regulators (PGR) towards in vitro regeneration and callus induction of new Malaysian rice variety of Oryza sativa L. var. MRIA 1. Intemode explants of 4-week old of aseptic seedlings were selected for in vitro regeneration and a total of 16 types of different combinations of NAA, IBA, BAP, and Kn were utilised. The most suitable plant growth regulators (PGR) for regeneration of Oryza sativa L. var. MRIA 1 was obtained from MS (Murashige and Skoog) media supplemented with 1.0 mg/L NAA and 0.5 mg/L Kn with an average of 8.90 ± 1.79 number of shoots per explant, 114.00 ± 18.90 number of roots per explant, 10.70 ± 2.62 cm length of shoots per explant and 8.70 ± 1.64 number of leaves per explant. The effect of combination of different plant growth regulators (PGR) supplemented in culture media on dry weight of regenerated Oryza sativa L. var. MRIA 1 were determined. Highest fresh weight (4.35±1.57 g) and dry weight (0.44±0.11 g per explant) recorded on the MS media supplemented with 1.0 mg/L NAA and 0.5 mg/L Kn. The callus inductions were assessed from three different explants including root, internode, and leaf base. The findings showed that the addition of 1.0 mg/L BAP into MS media was the most suitable for callus induction from root explant since it yield the highest fresh weight (1.039 ± 0.935 g per explant) and dry weight (0.093 ± 0.065 g per explant). The findings provided and optimum plant growth regulators (PGR) concentration for the tissue culture sysytem of Oryza sativa L. var. MRIA 1. Acclimatization of regenerated Oryza sativa L. var. MRIA 1 is recommended in order to serve a complete in vitro regeneration protocol of Oryza sativa L. var. MRIA 1.
format	Thesis
qualification_level	Master's degree
author	Mohamad Fauzi, Nor Yasmin
author_facet	Mohamad Fauzi, Nor Yasmin
author_sort	Mohamad Fauzi, Nor Yasmin
title	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_short	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_full	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_fullStr	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_full_unstemmed	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_sort	in vitro regeneration and callus induction of oryza sativa l. var. mria 1 / nor yasmin mohamad fauzi
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/27186/1/TM_NOR%20YASMIN%20MOHAMAD%20FAUZI%20AS%2016_5.pdf
_version_	1783733932227821568

In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi

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