Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria

Citrus grandis flowers possesses a strong floral-, jasmine - and orange- like aroma that has a potential in perfumery industries. Three extraction techniques that is hydrodistillation, Soxhlet extraction and solid phase micro extraction (SPME) were coupling with gas chromatograph -mass spectrometry...

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Main Author: Zakaria, Suhana
Format: Thesis
Language:English
Published: 2009
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Online Access:https://ir.uitm.edu.my/id/eprint/27593/1/TM_SUHANA%20ZAKARIA%20AS%2009_5.pdf
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spelling my-uitm-ir.275932020-01-22T05:35:20Z Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria 2009 Zakaria, Suhana Plant anatomy Plant physiology Citrus grandis flowers possesses a strong floral-, jasmine - and orange- like aroma that has a potential in perfumery industries. Three extraction techniques that is hydrodistillation, Soxhlet extraction and solid phase micro extraction (SPME) were coupling with gas chromatograph -mass spectrometry (GC-MS) for analysis of fragrance compounds from this flowers. About 120 compounds were detected in bud and blossom of these flowers, of which the five major fragrance compounds were β-myrcene, limonene, ocimene, linalool and caryophyllene. In hydrodistillation, ocimene and linalool detected in blossom were higher than in bud with 4.57%. However, Soxhlet extraction showed that bud contained high percent of limonene (27.25%), ocimene (5.55%) and linalool (2.97%) compared to blossom. Three different SPME fibers were used in this study, namely, 65 µm CAR/PDMS, 75 µm PDMS/DVB and 100 µm PDMS. The best result was obtained by 100 µM PDMS, that were then further analyzed for optimum sampling time. It showed 60 min fiber exposition time was the optimum sampling time to extract the major fragrance compounds from the headspace. In cell culture development, callus was successfully induced from different part of C. grandis flowers like petal , sepal, style, ovary, pistil and cup base on Murashige and Skoog (MS) medium supplemented with sucrose (30 g/l) and various concentrations of hormones. It was found that different parts of flowers required different level of hormone for callus induction. The highest formation of callus were obtained from petal and sepal cultured on MS media supplemented with 1.5 mg/l kinetin. For the style and pistil, 50% of the explants had developed callus when cultured on MS media added with 0.05 mg/l and 0.10 mg/l BAP respectively. On the other hand, sucrose agar media alone managed to induce callus formation from the cup base and ovary with success rate between 11 ± 1.29 to 25 ± 4.19%. Callus obtained were then subjected to SPME for determination of compounds in the callus. Seven compounds were detected in the callus which some of the compounds shows the same compounds as detected in the fresh flowers. 2009 Thesis https://ir.uitm.edu.my/id/eprint/27593/ https://ir.uitm.edu.my/id/eprint/27593/1/TM_SUHANA%20ZAKARIA%20AS%2009_5.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
topic Plant anatomy
Plant physiology
spellingShingle Plant anatomy
Plant physiology
Zakaria, Suhana
Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
description Citrus grandis flowers possesses a strong floral-, jasmine - and orange- like aroma that has a potential in perfumery industries. Three extraction techniques that is hydrodistillation, Soxhlet extraction and solid phase micro extraction (SPME) were coupling with gas chromatograph -mass spectrometry (GC-MS) for analysis of fragrance compounds from this flowers. About 120 compounds were detected in bud and blossom of these flowers, of which the five major fragrance compounds were β-myrcene, limonene, ocimene, linalool and caryophyllene. In hydrodistillation, ocimene and linalool detected in blossom were higher than in bud with 4.57%. However, Soxhlet extraction showed that bud contained high percent of limonene (27.25%), ocimene (5.55%) and linalool (2.97%) compared to blossom. Three different SPME fibers were used in this study, namely, 65 µm CAR/PDMS, 75 µm PDMS/DVB and 100 µm PDMS. The best result was obtained by 100 µM PDMS, that were then further analyzed for optimum sampling time. It showed 60 min fiber exposition time was the optimum sampling time to extract the major fragrance compounds from the headspace. In cell culture development, callus was successfully induced from different part of C. grandis flowers like petal , sepal, style, ovary, pistil and cup base on Murashige and Skoog (MS) medium supplemented with sucrose (30 g/l) and various concentrations of hormones. It was found that different parts of flowers required different level of hormone for callus induction. The highest formation of callus were obtained from petal and sepal cultured on MS media supplemented with 1.5 mg/l kinetin. For the style and pistil, 50% of the explants had developed callus when cultured on MS media added with 0.05 mg/l and 0.10 mg/l BAP respectively. On the other hand, sucrose agar media alone managed to induce callus formation from the cup base and ovary with success rate between 11 ± 1.29 to 25 ± 4.19%. Callus obtained were then subjected to SPME for determination of compounds in the callus. Seven compounds were detected in the callus which some of the compounds shows the same compounds as detected in the fresh flowers.
format Thesis
qualification_level Master's degree
author Zakaria, Suhana
author_facet Zakaria, Suhana
author_sort Zakaria, Suhana
title Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_short Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_full Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_fullStr Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_full_unstemmed Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_sort compounds and development of cell cultures of citrus grandis (osbeck.) flowers / suhana zakaria
granting_institution Universiti Teknologi MARA
granting_department Faculty of Applied Sciences
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/27593/1/TM_SUHANA%20ZAKARIA%20AS%2009_5.pdf
_version_ 1783733979516502016