Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun

Introduction: Pitfall is known as misdiagnosing or misinterpretation that can lead to whether false positive or false negative result, and may affect treatment, thus lead to morbidity. Error in misdiagnosis can contribute to false treatment and may lead to death. Possible contaminants in cytomorphol...

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Main Author:	Harun, Nur Sakinah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Cytology
Online Access:	https://ir.uitm.edu.my/id/eprint/28082/1/TD_NUR%20SAKINAH%20%20HARUN%20HS%2015_5.pdf
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spelling	my-uitm-ir.280822020-02-03T04:32:25Z Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun 2015 Harun, Nur Sakinah Cytology Introduction: Pitfall is known as misdiagnosing or misinterpretation that can lead to whether false positive or false negative result, and may affect treatment, thus lead to morbidity. Error in misdiagnosis can contribute to false treatment and may lead to death. Possible contaminants in cytomorphological study, including food particles such as fruits and grains may cause pitfall by mimicking certain human cells or fungal element that present in human, and staining background. Objectives: Purposes of this study are to identify cytomorphology of tropical fruits and local grains, which may cause pitfalls in cytological diagnosis, and to compare and recognize the mimicking morphological characteristics of the selected fruits and grains cells to cells in cytology. Method: Random tropical fruits and local grains were selected as samples. The samples were homogenized using mortar and pestle, and smeared onto glass slides. For each sample, two slides were prepared, one slide was fixed into 95% alcohol, and another one was air-dried. The slides were then stained using Papanicolaou and May Grunwald's Giemsa respectively. Stained slides were observed for cells morphologies and background. Selected cells morphologies were then captured using Leica DM750 Microscope with ICC 50HD camera. Results: Dragon fruit cells showed resembling criteria of benign lipoma and squamous epithelial cell, where mango fruit cells resemble cryptococcosis and fat droplet in adipocyte tissue. Ambarella fruit cells showed 'honeycomb' characteristics of endocervical cell. Starfruit cells and watermelon fruit cells showed resemblances to squamous epithelial cell and hyperkeratosis respectively, where maize cells resemble haematoxylin scum. Conclusion: The morphological characteristics of tropical fruits and local grains can resemble morphology of human cells and staining background. These findings can be used as references in cytological diagnosis, thus avoiding the potential pitfalls. 2015 Thesis https://ir.uitm.edu.my/id/eprint/28082/ https://ir.uitm.edu.my/id/eprint/28082/1/TD_NUR%20SAKINAH%20%20HARUN%20HS%2015_5.pdf text en public degree Universiti Teknologi MARA Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Cytology
spellingShingle	Cytology Harun, Nur Sakinah Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun
description	Introduction: Pitfall is known as misdiagnosing or misinterpretation that can lead to whether false positive or false negative result, and may affect treatment, thus lead to morbidity. Error in misdiagnosis can contribute to false treatment and may lead to death. Possible contaminants in cytomorphological study, including food particles such as fruits and grains may cause pitfall by mimicking certain human cells or fungal element that present in human, and staining background. Objectives: Purposes of this study are to identify cytomorphology of tropical fruits and local grains, which may cause pitfalls in cytological diagnosis, and to compare and recognize the mimicking morphological characteristics of the selected fruits and grains cells to cells in cytology. Method: Random tropical fruits and local grains were selected as samples. The samples were homogenized using mortar and pestle, and smeared onto glass slides. For each sample, two slides were prepared, one slide was fixed into 95% alcohol, and another one was air-dried. The slides were then stained using Papanicolaou and May Grunwald's Giemsa respectively. Stained slides were observed for cells morphologies and background. Selected cells morphologies were then captured using Leica DM750 Microscope with ICC 50HD camera. Results: Dragon fruit cells showed resembling criteria of benign lipoma and squamous epithelial cell, where mango fruit cells resemble cryptococcosis and fat droplet in adipocyte tissue. Ambarella fruit cells showed 'honeycomb' characteristics of endocervical cell. Starfruit cells and watermelon fruit cells showed resemblances to squamous epithelial cell and hyperkeratosis respectively, where maize cells resemble haematoxylin scum. Conclusion: The morphological characteristics of tropical fruits and local grains can resemble morphology of human cells and staining background. These findings can be used as references in cytological diagnosis, thus avoiding the potential pitfalls.
format	Thesis
qualification_level	Bachelor degree
author	Harun, Nur Sakinah
author_facet	Harun, Nur Sakinah
author_sort	Harun, Nur Sakinah
title	Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun
title_short	Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun
title_full	Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun
title_fullStr	Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun
title_full_unstemmed	Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun
title_sort	potential pitfall in cytological diagnosis: cytomorphology of tropical fruits and local grains in malaysia / nur sakinah harun
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Health Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/28082/1/TD_NUR%20SAKINAH%20%20HARUN%20HS%2015_5.pdf
_version_	1783734048812695552

Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun

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