Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor

Micropropagation of banana is well known and the product derived from it is accepted among banana plantation community. Tissue culture banana basically having better characteristics than banana plant resulting from conventional cultivation. It is due to the plant selection where the plant having muc...

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Main Author:	Mohd Nor, Mohd Sukri
Format:	Thesis
Language:	English
Published:	2020
Subjects:	Biology Musaceae (Banana) QP Physiology
Online Access:	https://ir.uitm.edu.my/id/eprint/32406/1/32406.pdf
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id	my-uitm-ir.32406
record_format	uketd_dc
spelling	my-uitm-ir.324062024-09-12T04:40:36Z Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor 2020 Mohd Nor, Mohd Sukri Biology Musaceae (Banana) QP Physiology Cultures and culture media. Culture technique. Micropropagation of banana is well known and the product derived from it is accepted among banana plantation community. Tissue culture banana basically having better characteristics than banana plant resulting from conventional cultivation. It is due to the plant selection where the plant having much better quality will likely to be choose. With the help of micropropagation technique, it is feasible to produce hundreds to thousands of planting materials with super-trait which is exactly the same as mother plant. In Malaysia, tissue culture technique for several banana cultivar has been established. Unfortunately, it is not for banana known as Tanduk. Problems surrounding tissue culture of Musa paradisiaca cv. Tanduk is mainly due to its high phenolic content which will leads to browning. Unsolved browning issue will cause the death of the plant. In this study, minimization of browning is our concern where micropropagation of Musa paradisiaca cv. Tanduk were subjected with three (3) different treatments: 1) Ascorbic acid 0.0 – 20.0 mg/L; 2) Photoperiod of 16 hours light and 8 hours dark, and 24 hours darkness condition; 3) Half and full medium strength. The result shows that 15.0 mg/L ascorbic acid in a 16 hours light and 8 hours darkness photoperiod were observed to effectively minimize browning in the initiation stage. It is supported by PPO Assay data conducted simultaneously. Finally, variation among the treated banana plants were observed morphologically and the observation conducted shows insignificant variation among the treated banana plants. 2020 Thesis https://ir.uitm.edu.my/id/eprint/32406/ https://ir.uitm.edu.my/id/eprint/32406/1/32406.pdf text en public masters Universiti Teknologi MARA Faculty of Plantation & Agrotechnology Awal, Asmah Khalid, Norzulaani
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Awal, Asmah Khalid, Norzulaani
topic	Biology Musaceae (Banana) QP Physiology Biology
spellingShingle	Biology Musaceae (Banana) QP Physiology Biology Mohd Nor, Mohd Sukri Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor
description	Micropropagation of banana is well known and the product derived from it is accepted among banana plantation community. Tissue culture banana basically having better characteristics than banana plant resulting from conventional cultivation. It is due to the plant selection where the plant having much better quality will likely to be choose. With the help of micropropagation technique, it is feasible to produce hundreds to thousands of planting materials with super-trait which is exactly the same as mother plant. In Malaysia, tissue culture technique for several banana cultivar has been established. Unfortunately, it is not for banana known as Tanduk. Problems surrounding tissue culture of Musa paradisiaca cv. Tanduk is mainly due to its high phenolic content which will leads to browning. Unsolved browning issue will cause the death of the plant. In this study, minimization of browning is our concern where micropropagation of Musa paradisiaca cv. Tanduk were subjected with three (3) different treatments: 1) Ascorbic acid 0.0 – 20.0 mg/L; 2) Photoperiod of 16 hours light and 8 hours dark, and 24 hours darkness condition; 3) Half and full medium strength. The result shows that 15.0 mg/L ascorbic acid in a 16 hours light and 8 hours darkness photoperiod were observed to effectively minimize browning in the initiation stage. It is supported by PPO Assay data conducted simultaneously. Finally, variation among the treated banana plants were observed morphologically and the observation conducted shows insignificant variation among the treated banana plants.
format	Thesis
qualification_level	Master's degree
author	Mohd Nor, Mohd Sukri
author_facet	Mohd Nor, Mohd Sukri
author_sort	Mohd Nor, Mohd Sukri
title	Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor
title_short	Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor
title_full	Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor
title_fullStr	Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor
title_full_unstemmed	Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor
title_sort	browning inhibition in in vitro regeneration of musa paradisiaca cv. tanduk using ascorbic acid / mohd sukri mohd nor
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Plantation & Agrotechnology
publishDate	2020
url	https://ir.uitm.edu.my/id/eprint/32406/1/32406.pdf
_version_	1811768605275062272

Browning inhibition in in vitro regeneration of Musa Paradisiaca cv. tanduk using ascorbic acid / Mohd Sukri Mohd Nor

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