Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud

Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud

In this work, nanoporous anodic aluminium oxide (AAO) membrane-based optical biosensor was constructed for targeting the DNA Dengue virus type II. The AAO membrane was fabricated by electrochemical anodization of aluminium foil applying different voltages from 40-100 V. To increase the sensitivity o...

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Main Author: Mahmud, Abdul Hadi
Format: Thesis
Language:English
Published: 2017
Subjects:
Nanoparticles
Online Access:https://ir.uitm.edu.my/id/eprint/37236/1/37236.pdf
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spelling my-uitm-ir.372362023-08-21T02:44:36Z Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud 2017 Mahmud, Abdul Hadi Nanoparticles In this work, nanoporous anodic aluminium oxide (AAO) membrane-based optical biosensor was constructed for targeting the DNA Dengue virus type II. The AAO membrane was fabricated by electrochemical anodization of aluminium foil applying different voltages from 40-100 V. To increase the sensitivity of the biosensor device, amino (l-propyl-4,5-dihydro-lH-l,2,3-triazol-4-yi)-triethoxysilane (click compound) was synthesized and introduced on to the AAO by silanization approach followed by l-ethyl-3-[3-dimethylaminopropyl] carbodiimide (EDC) mediated immobilization of amino (Nty-modified probe ssDNA. Fluorescent-tagged (Texas red) complementary target DNA was used for DNA hybridization process on modified AAO. Characterization of the AAO membrane's morphology was observed by means of Field Emission Scanning Electron Microscope (FE-SEM) 2017 Thesis https://ir.uitm.edu.my/id/eprint/37236/ https://ir.uitm.edu.my/id/eprint/37236/1/37236.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Md Jani, Abdul Mutalib
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Md Jani, Abdul Mutalib
topic Nanoparticles
spellingShingle Nanoparticles
Mahmud, Abdul Hadi
Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud
description In this work, nanoporous anodic aluminium oxide (AAO) membrane-based optical biosensor was constructed for targeting the DNA Dengue virus type II. The AAO membrane was fabricated by electrochemical anodization of aluminium foil applying different voltages from 40-100 V. To increase the sensitivity of the biosensor device, amino (l-propyl-4,5-dihydro-lH-l,2,3-triazol-4-yi)-triethoxysilane (click compound) was synthesized and introduced on to the AAO by silanization approach followed by l-ethyl-3-[3-dimethylaminopropyl] carbodiimide (EDC) mediated immobilization of amino (Nty-modified probe ssDNA. Fluorescent-tagged (Texas red) complementary target DNA was used for DNA hybridization process on modified AAO. Characterization of the AAO membrane's morphology was observed by means of Field Emission Scanning Electron Microscope (FE-SEM)
format Thesis
qualification_level Master's degree
author Mahmud, Abdul Hadi
author_facet Mahmud, Abdul Hadi
author_sort Mahmud, Abdul Hadi
title Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud
title_short Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud
title_full Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud
title_fullStr Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud
title_full_unstemmed Surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type II / Abdul Hadi Mahmud
title_sort surface engineering of nanoporous anodic aluminium oxide for biosensor application in the detection of dengue virus type ii / abdul hadi mahmud
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Applied Sciences
publishDate 2017
url https://ir.uitm.edu.my/id/eprint/37236/1/37236.pdf
_version_ 1783734407168786432

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