Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa

Melastoma decemfidum {M. decemfidum) is a medicinal plant that belongs to the family Melastomataceae. It can grow wild, but difficult to find as compared to other species of Melastomataceae. This plant was reported to produce bioactive flavonoids, having antimicrobial activities. The flavonoid biosy...

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Main Author:	Che Isa, Nurul Huda
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Clinical pathology Laboratory technique
Online Access:	https://ir.uitm.edu.my/id/eprint/38892/1/38892.pdf
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id	my-uitm-ir.38892
record_format	uketd_dc
spelling	my-uitm-ir.388922024-09-23T06:24:21Z Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa 2017-03 Che Isa, Nurul Huda Clinical pathology. Laboratory technique Melastoma decemfidum {M. decemfidum) is a medicinal plant that belongs to the family Melastomataceae. It can grow wild, but difficult to find as compared to other species of Melastomataceae. This plant was reported to produce bioactive flavonoids, having antimicrobial activities. The flavonoid biosynthesis pathway involves a key enzyme called chalcone synthase (CHS). The present study was aimed to determine the antimicrobial activities of different concentrations of extracts and to isolate and amplify the CHS gene from in vitro M. decemfidum plant. The antimicrobial activities were determined using agar disc diffusion method against tested microorganisms which include Gram positive bacteria {Staphylococcus aureus), Gram negative bacteria {Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium) and fungi {Candida albicans and Aspergillus brasiliensis). Genomic DNA was extracted and a partial CHS gene was amplified using designed primers. Finding showed that S. aureus was the most sensitive towards leaves and whole plant extracts, while A. brasiliensis were resistant to both extracts (leaves and whole plant extract). The range of MIC values for the leaves and whole plant extracts were 50 to 200 mg/ml. While the MBC and MFC range from 100 to 200 mg/ml. The amplified PCR products with the approximate size of 136 and 140 nucleotide base pairs were obtained from leaves and whole plant extract. This is the first report to describe the isolation of CHS gene from in vitro M. decemfidum. 2017-03 Thesis https://ir.uitm.edu.my/id/eprint/38892/ https://ir.uitm.edu.my/id/eprint/38892/1/38892.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences Wan Abdul Razak, Wan Razarinah (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Wan Abdul Razak, Wan Razarinah (Dr.)
topic	Clinical pathology Laboratory technique
spellingShingle	Clinical pathology Laboratory technique Che Isa, Nurul Huda Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa
description	Melastoma decemfidum {M. decemfidum) is a medicinal plant that belongs to the family Melastomataceae. It can grow wild, but difficult to find as compared to other species of Melastomataceae. This plant was reported to produce bioactive flavonoids, having antimicrobial activities. The flavonoid biosynthesis pathway involves a key enzyme called chalcone synthase (CHS). The present study was aimed to determine the antimicrobial activities of different concentrations of extracts and to isolate and amplify the CHS gene from in vitro M. decemfidum plant. The antimicrobial activities were determined using agar disc diffusion method against tested microorganisms which include Gram positive bacteria {Staphylococcus aureus), Gram negative bacteria {Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium) and fungi {Candida albicans and Aspergillus brasiliensis). Genomic DNA was extracted and a partial CHS gene was amplified using designed primers. Finding showed that S. aureus was the most sensitive towards leaves and whole plant extracts, while A. brasiliensis were resistant to both extracts (leaves and whole plant extract). The range of MIC values for the leaves and whole plant extracts were 50 to 200 mg/ml. While the MBC and MFC range from 100 to 200 mg/ml. The amplified PCR products with the approximate size of 136 and 140 nucleotide base pairs were obtained from leaves and whole plant extract. This is the first report to describe the isolation of CHS gene from in vitro M. decemfidum.
format	Thesis
qualification_level	Master's degree
author	Che Isa, Nurul Huda
author_facet	Che Isa, Nurul Huda
author_sort	Che Isa, Nurul Huda
title	Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa
title_short	Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa
title_full	Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa
title_fullStr	Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa
title_full_unstemmed	Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa
title_sort	antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / nurul huda che isa
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/38892/1/38892.pdf
_version_	1811768623874703360

Antimicrobial activity of in vitro melastoma decemfidum plant extract and isolation of partial chalcone synthase gene / Nurul Huda Che Isa

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