Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding

Winged bean {Psophocarpus tetragonolobus) seed protein hydrolysates, green soy bean {Glycine max L) seed protein hydrolysates and sea cucumber (Stichopus horrens) protein hydrolysate were known as a potential source for generating peptides with biological activities such as angiotensin-converting en...

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Main Author:	Kawanding, Obren James
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Conditions and laws of chemical reactions
Online Access:	https://ir.uitm.edu.my/id/eprint/39268/1/39268.pdf
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spelling	my-uitm-ir.392682021-08-30T02:19:23Z Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding 2017-01 Kawanding, Obren James Conditions and laws of chemical reactions Winged bean {Psophocarpus tetragonolobus) seed protein hydrolysates, green soy bean {Glycine max L) seed protein hydrolysates and sea cucumber (Stichopus horrens) protein hydrolysate were known as a potential source for generating peptides with biological activities such as angiotensin-converting enzyme (ACE) inhibitory which act as an anti-hypertensive agent. Despite the functional properties of protein hydrolysate, there was lack of information on the safety of the hydrolysates. The safety of winged bean, green soy bean protein hydrolysates and sea cucumber (Stichopus horrens) protein hydrolysate has been confirmed in several in vitro studies including in vitro genotoxicity tests (Ames test and in vitro micronucleus test) and cytotoxicity test (neutral red uptake assay). Mutant strains of Salmonella typhimurium bacteria were used in Ames test to evaluate the mutagenicity effect of the protein hydrolysate samples while V79 Chinese hamster lung fibroblast cell was used to evaluate the clastogenicity effect of the hydrolysate samples. NIH/3T3 cell embryonic mouse fibroblast cell was used to determine the cytotoxic effect of the hydrolysate samples. The mutagenicity test, clastogenicity test and cytotoxicity test confirmed that winged bean, green soy bean and sea cucumber protein hydrolysates was not mutagenic, clastogenic and cytotoxic respectively. In conclusion, winged bean and green soy bean protein hydrolysate is safe in term of mutagenicity, clastogenicity and cytotoxicity. However, several tests including in vivo and pre-clinical study should be conducted in order to verify the safety of the protein hydrolysate samples so that the protein hydrolysate can be commercialized for human benefits. 2017-01 Thesis https://ir.uitm.edu.my/id/eprint/39268/ https://ir.uitm.edu.my/id/eprint/39268/1/39268.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences Abdullah, Mohd Faiz Foong (Assoc. Prof. Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdullah, Mohd Faiz Foong (Assoc. Prof. Dr.)
topic	Conditions and laws of chemical reactions
spellingShingle	Conditions and laws of chemical reactions Kawanding, Obren James Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding
description	Winged bean {Psophocarpus tetragonolobus) seed protein hydrolysates, green soy bean {Glycine max L) seed protein hydrolysates and sea cucumber (Stichopus horrens) protein hydrolysate were known as a potential source for generating peptides with biological activities such as angiotensin-converting enzyme (ACE) inhibitory which act as an anti-hypertensive agent. Despite the functional properties of protein hydrolysate, there was lack of information on the safety of the hydrolysates. The safety of winged bean, green soy bean protein hydrolysates and sea cucumber (Stichopus horrens) protein hydrolysate has been confirmed in several in vitro studies including in vitro genotoxicity tests (Ames test and in vitro micronucleus test) and cytotoxicity test (neutral red uptake assay). Mutant strains of Salmonella typhimurium bacteria were used in Ames test to evaluate the mutagenicity effect of the protein hydrolysate samples while V79 Chinese hamster lung fibroblast cell was used to evaluate the clastogenicity effect of the hydrolysate samples. NIH/3T3 cell embryonic mouse fibroblast cell was used to determine the cytotoxic effect of the hydrolysate samples. The mutagenicity test, clastogenicity test and cytotoxicity test confirmed that winged bean, green soy bean and sea cucumber protein hydrolysates was not mutagenic, clastogenic and cytotoxic respectively. In conclusion, winged bean and green soy bean protein hydrolysate is safe in term of mutagenicity, clastogenicity and cytotoxicity. However, several tests including in vivo and pre-clinical study should be conducted in order to verify the safety of the protein hydrolysate samples so that the protein hydrolysate can be commercialized for human benefits.
format	Thesis
qualification_level	Master's degree
author	Kawanding, Obren James
author_facet	Kawanding, Obren James
author_sort	Kawanding, Obren James
title	Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding
title_short	Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding
title_full	Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding
title_fullStr	Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding
title_full_unstemmed	Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding
title_sort	genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / obren james kawanding
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/39268/1/39268.pdf
_version_	1783734499216982016

Genotoxicity and cytotoxicity profiles of winged bean, green soybean and sea cucumber protein hydrolysates / Obren James Kawanding

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