In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar

Pogostemon cablin is an important aromatic plant producing patchouli oil from its leaves' extraction. The main objective of this study was to develop an effective method for in vitro propagation of Malaysian cultivated P. cablin. The effect of varying the strength of MS medium was investigated...

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Main Author:	Wan Anuar, Wan Nurul Hidayah
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Physical and theoretical chemistry Analytical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/39686/1/39686.pdf
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id	my-uitm-ir.39686
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spelling	my-uitm-ir.396862022-12-06T08:50:33Z In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar 2014-04 Wan Anuar, Wan Nurul Hidayah Physical and theoretical chemistry Analytical chemistry Pogostemon cablin is an important aromatic plant producing patchouli oil from its leaves' extraction. The main objective of this study was to develop an effective method for in vitro propagation of Malaysian cultivated P. cablin. The effect of varying the strength of MS medium was investigated using node explants. Different concentrations of BAP and NAA were added to both full and half strength MS (Murashige and Skoog) medium for regeneration of this plant. The MS media supplemented with 0.25 mg/1 BAP gave the highest number of shoots and length followed by the combinations of hormones 1.0 mg/1 BAP and 0.25 mg/1 NAA. The in vitro plantlets were then acclimatized in two different substrates that were soil and the soil mixture with vermiculite which was found with 100% survival rate obtained by in vitro grown P. cablin plants acclimatized in soil. The leaves part were harvested from both sources of in vitro and grown plants and ex vitro mother plant of P. cablin for further extraction of its essential oil via hydrodistillation and then were analyzed using GC-MS. The gas chromatogram of the extracted oils from both samples showed similar essential oil profiles with relative amount of patchouli alcohol were higher in the in vitro grown plants than that in ex vitro mother plant. The monomorphic banding pattern analyzed using two universal primers, cytochrome c oxidase (coxl) and maturase K (matK) indicated that the true to type plants of P. cablin were established from in vitro propagation through direct propagation from node explants. Rapid and high multiplication frequency as well as the essential oil content ensures the efficacy of the protocol developed for the production of this industrially important aromatic plant. 2014-04 Thesis https://ir.uitm.edu.my/id/eprint/39686/ https://ir.uitm.edu.my/id/eprint/39686/1/39686.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences Jaafar Sidik, Norrizah (Dr )
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Jaafar Sidik, Norrizah (Dr )
topic	Physical and theoretical chemistry Analytical chemistry
spellingShingle	Physical and theoretical chemistry Analytical chemistry Wan Anuar, Wan Nurul Hidayah In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar
description	Pogostemon cablin is an important aromatic plant producing patchouli oil from its leaves' extraction. The main objective of this study was to develop an effective method for in vitro propagation of Malaysian cultivated P. cablin. The effect of varying the strength of MS medium was investigated using node explants. Different concentrations of BAP and NAA were added to both full and half strength MS (Murashige and Skoog) medium for regeneration of this plant. The MS media supplemented with 0.25 mg/1 BAP gave the highest number of shoots and length followed by the combinations of hormones 1.0 mg/1 BAP and 0.25 mg/1 NAA. The in vitro plantlets were then acclimatized in two different substrates that were soil and the soil mixture with vermiculite which was found with 100% survival rate obtained by in vitro grown P. cablin plants acclimatized in soil. The leaves part were harvested from both sources of in vitro and grown plants and ex vitro mother plant of P. cablin for further extraction of its essential oil via hydrodistillation and then were analyzed using GC-MS. The gas chromatogram of the extracted oils from both samples showed similar essential oil profiles with relative amount of patchouli alcohol were higher in the in vitro grown plants than that in ex vitro mother plant. The monomorphic banding pattern analyzed using two universal primers, cytochrome c oxidase (coxl) and maturase K (matK) indicated that the true to type plants of P. cablin were established from in vitro propagation through direct propagation from node explants. Rapid and high multiplication frequency as well as the essential oil content ensures the efficacy of the protocol developed for the production of this industrially important aromatic plant.
format	Thesis
qualification_level	Master's degree
author	Wan Anuar, Wan Nurul Hidayah
author_facet	Wan Anuar, Wan Nurul Hidayah
author_sort	Wan Anuar, Wan Nurul Hidayah
title	In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar
title_short	In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar
title_full	In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar
title_fullStr	In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar
title_full_unstemmed	In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar
title_sort	in vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / wan nurul hidayah wan anuar
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2014
url	https://ir.uitm.edu.my/id/eprint/39686/1/39686.pdf
_version_	1783734529349910528

In vitro propagation and comparative study on phytochemical profiles of pogostemon cablin / Wan Nurul Hidayah Wan Anuar

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