Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin

Soursop (Annona muricata) is known for its ethnomedicinal uses, leading to the increasing demand for the fruits. In Malaysia, leaf spot disease is one of the major constraints in the soursop plantation. If unattended, the possibility for the disease to cause huge economic losses in the soursop indus...

Full description

Saved in:

Bibliographic Details
Main Author:	Muslimin, Nurul Syafika
Format:	Thesis
Language:	English
Published:	2023
Subjects:	Medicine Folk medicine QK Botany
Online Access:	https://ir.uitm.edu.my/id/eprint/42430/6/42430.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.42430
record_format	uketd_dc
spelling	my-uitm-ir.424302024-07-22T06:34:11Z Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin 2023 Muslimin, Nurul Syafika Medicine. Folk medicine QK Botany Soursop (Annona muricata) is known for its ethnomedicinal uses, leading to the increasing demand for the fruits. In Malaysia, leaf spot disease is one of the major constraints in the soursop plantation. If unattended, the possibility for the disease to cause huge economic losses in the soursop industry are highly possible. Thus, this study aimed to determine the morphological characteristic of the isolated fungi associated with leaf spot disease of soursop, to assess molecular identification of the isolated fungi by amplification of ITS regions, to analyse the antagonistic activity of Trichoderma harzianum against identified fungal isolates by using an application of dual culture test in vitro, to determine the severity of infection of identified pathogenic fungi on the soursop saplings in vivo, and to determine the capability of T. harzianum as plant growth-promoting fungi (PGPF) on soursop saplings in vivo. The leaves and soil samples from the infected soursop trees with leaf spot disease symptoms were collected from the farms located in Seri Menanti, Negeri Sembilan and Bukit Kurau, Perak, Malaysia. Cultivation was done on Potato Dextrose Agar (PDA) medium. The morphology of the fungal isolates was identified using fungal slide culture method Then, further identification for the fungal species was done molecularly by amplification of ITS region. Antagonistic activity of T. harzianum against fungal isolates was identified using dual culture method and its capability as plant growth - promoting fungus was done by application of T. harzianum suspension on soursop saplings. From the results, twenty-six morphologically distinct fungi isolates were identified and only 12 fungal isolates were suspected to be pathogenic. 2023 Thesis https://ir.uitm.edu.my/id/eprint/42430/ https://ir.uitm.edu.my/id/eprint/42430/6/42430.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Mohd Yusof, Farida Zuraina Abdul Khalil, Khalilah Boll Khassim, Qursyna
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mohd Yusof, Farida Zuraina Abdul Khalil, Khalilah Boll Khassim, Qursyna
topic	Medicine Folk medicine QK Botany
spellingShingle	Medicine Folk medicine QK Botany Muslimin, Nurul Syafika Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin
description	Soursop (Annona muricata) is known for its ethnomedicinal uses, leading to the increasing demand for the fruits. In Malaysia, leaf spot disease is one of the major constraints in the soursop plantation. If unattended, the possibility for the disease to cause huge economic losses in the soursop industry are highly possible. Thus, this study aimed to determine the morphological characteristic of the isolated fungi associated with leaf spot disease of soursop, to assess molecular identification of the isolated fungi by amplification of ITS regions, to analyse the antagonistic activity of Trichoderma harzianum against identified fungal isolates by using an application of dual culture test in vitro, to determine the severity of infection of identified pathogenic fungi on the soursop saplings in vivo, and to determine the capability of T. harzianum as plant growth-promoting fungi (PGPF) on soursop saplings in vivo. The leaves and soil samples from the infected soursop trees with leaf spot disease symptoms were collected from the farms located in Seri Menanti, Negeri Sembilan and Bukit Kurau, Perak, Malaysia. Cultivation was done on Potato Dextrose Agar (PDA) medium. The morphology of the fungal isolates was identified using fungal slide culture method Then, further identification for the fungal species was done molecularly by amplification of ITS region. Antagonistic activity of T. harzianum against fungal isolates was identified using dual culture method and its capability as plant growth - promoting fungus was done by application of T. harzianum suspension on soursop saplings. From the results, twenty-six morphologically distinct fungi isolates were identified and only 12 fungal isolates were suspected to be pathogenic.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Muslimin, Nurul Syafika
author_facet	Muslimin, Nurul Syafika
author_sort	Muslimin, Nurul Syafika
title	Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin
title_short	Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin
title_full	Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin
title_fullStr	Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin
title_full_unstemmed	Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin
title_sort	biological control of fungi associates in soursop (annona muricata) leaf spot disease by using trichoderma harzianum / nurul syafika muslimin
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2023
url	https://ir.uitm.edu.my/id/eprint/42430/6/42430.pdf
_version_	1811768630860316672

Biological control of fungi associates in soursop (Annona muricata) leaf spot disease by using Trichoderma harzianum / Nurul Syafika Muslimin

Similar Items