Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh

Formalin fixed samples become a valuable tool for detection of gene expression marker upon diagnosing a diseases. The used of 10 % formalin become a gold standard for histopathological analysis but the effect of formalin fixation in prolong tissue storage influence the credibility of the molecular m...

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Main Author:	Suboh, Asiah Mohd
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Genetics Organic substances
Online Access:	https://ir.uitm.edu.my/id/eprint/50076/1/50076.pdf
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id	my-uitm-ir.50076
record_format	uketd_dc
spelling	my-uitm-ir.500762021-09-06T06:20:58Z Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh 2017 Suboh, Asiah Mohd Genetics Organic substances Formalin fixed samples become a valuable tool for detection of gene expression marker upon diagnosing a diseases. The used of 10 % formalin become a gold standard for histopathological analysis but the effect of formalin fixation in prolong tissue storage influence the credibility of the molecular marker to be extracted. In addition, with the characteristic of molecular marker that easily degrading becomes a challenging step to obtain pure marker. As formalin used to preserve the morphology of tissue, their effectiveness to preserve tissue and also the molecular compartment in the tissue were studied. Two approaches were taken to determine the association of tissue morphology with RNA and miRNA quality. Samples undergo histology processing and visualization under light microscope before being proceed with RNA extraction and quantification with Eppendorf Biophotometer Plus and Agilent 2100 bioanalyser to evaluate the quantity and quality of isolated material. The morphology of all samples were generally similar even though from different year which showed marked changes of cellular characteristics displayed as;- elongated, loss of nucleus and faded cytoplasm border. It then supported with the RNA and miRNA concentration present, which showed low level for both years. Eventhough the quality and quantity of both markers were unsatisfied, but the availability of those markers in long-term tissue storage is still accessible. The morphological effects of formalin fixed sample do have associated with integrity of both RNA and miRNA as the result are in linear correlation. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50076/ https://ir.uitm.edu.my/id/eprint/50076/1/50076.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Mustakim, Maimunah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mustakim, Maimunah
topic	Genetics Organic substances
spellingShingle	Genetics Organic substances Suboh, Asiah Mohd Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh
description	Formalin fixed samples become a valuable tool for detection of gene expression marker upon diagnosing a diseases. The used of 10 % formalin become a gold standard for histopathological analysis but the effect of formalin fixation in prolong tissue storage influence the credibility of the molecular marker to be extracted. In addition, with the characteristic of molecular marker that easily degrading becomes a challenging step to obtain pure marker. As formalin used to preserve the morphology of tissue, their effectiveness to preserve tissue and also the molecular compartment in the tissue were studied. Two approaches were taken to determine the association of tissue morphology with RNA and miRNA quality. Samples undergo histology processing and visualization under light microscope before being proceed with RNA extraction and quantification with Eppendorf Biophotometer Plus and Agilent 2100 bioanalyser to evaluate the quantity and quality of isolated material. The morphology of all samples were generally similar even though from different year which showed marked changes of cellular characteristics displayed as;- elongated, loss of nucleus and faded cytoplasm border. It then supported with the RNA and miRNA concentration present, which showed low level for both years. Eventhough the quality and quantity of both markers were unsatisfied, but the availability of those markers in long-term tissue storage is still accessible. The morphological effects of formalin fixed sample do have associated with integrity of both RNA and miRNA as the result are in linear correlation.
format	Thesis
qualification_level	Bachelor degree
author	Suboh, Asiah Mohd
author_facet	Suboh, Asiah Mohd
author_sort	Suboh, Asiah Mohd
title	Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh
title_short	Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh
title_full	Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh
title_fullStr	Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh
title_full_unstemmed	Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh
title_sort	determination of tissue morphology and rna integrity from rabbit’s heart archive sample in long term tissue storage / asiah mohd suboh
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50076/1/50076.pdf
_version_	1783734818970796032

Determination of tissue morphology and RNA integrity from rabbit’s heart archive sample in long term tissue storage / Asiah Mohd Suboh

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