Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor

Raw vegetables or ulam are one of essential diets of human being and become a side dish in a daily plate. But, all these salad vegetables might be contaminated with pathogenic bacteria that can caused food borne illness. Three different types of ulam were selected from two different retail outlet lo...

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Main Author:	Mohd Nor, Izzafaiqaainaa
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Bacteria Vegetables
Online Access:	https://ir.uitm.edu.my/id/eprint/50143/1/50143.pdf
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id	my-uitm-ir.50143
record_format	uketd_dc
spelling	my-uitm-ir.501432021-09-07T01:31:26Z Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor 2017 Mohd Nor, Izzafaiqaainaa Bacteria Antibiotic therapy. Antibiotics Vegetables Raw vegetables or ulam are one of essential diets of human being and become a side dish in a daily plate. But, all these salad vegetables might be contaminated with pathogenic bacteria that can caused food borne illness. Three different types of ulam were selected from two different retail outlet located in Puncak Alam, Selangor and their bacteriological attributes were examined by using routine microbiological method. Twenty one of bacteria isolated from ulam were further investigated by using, polymerase chain reaction (PCR) assay targeting 16s rDNA gene. Total viable count (TVC) and total coliform count (TCC) in all three samples were both too numerous to count (TNTC) .The highest total staphylococcal count (TSC) was found in selom (4.0 x 106) followed by lettuce (3.8 x 106) and kacang botol (2.0 x 106). Twenty one bacterial isolates from three ulam samples have both gram positive and gram negative bacteria. More than four predominating bacteria are detected in the isolates which are Enterobacter spp., Citrobacter spp., Klebsiella spp., Staphylococcus aureus, Acinetobacter spp., and Bacillus spp. Bacterial identification by using 16s rDNA primer showed only Staphylococcus aureus was identified from the studied of bacterial isolates. Proper transportation, hygiene handling and proper storage are essential to prevent cross contamination of pathogenic bacteria in salad vegetables. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50143/ https://ir.uitm.edu.my/id/eprint/50143/1/50143.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Mustakim, Maimunah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mustakim, Maimunah
topic	Bacteria Bacteria Vegetables
spellingShingle	Bacteria Bacteria Vegetables Mohd Nor, Izzafaiqaainaa Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor
description	Raw vegetables or ulam are one of essential diets of human being and become a side dish in a daily plate. But, all these salad vegetables might be contaminated with pathogenic bacteria that can caused food borne illness. Three different types of ulam were selected from two different retail outlet located in Puncak Alam, Selangor and their bacteriological attributes were examined by using routine microbiological method. Twenty one of bacteria isolated from ulam were further investigated by using, polymerase chain reaction (PCR) assay targeting 16s rDNA gene. Total viable count (TVC) and total coliform count (TCC) in all three samples were both too numerous to count (TNTC) .The highest total staphylococcal count (TSC) was found in selom (4.0 x 106) followed by lettuce (3.8 x 106) and kacang botol (2.0 x 106). Twenty one bacterial isolates from three ulam samples have both gram positive and gram negative bacteria. More than four predominating bacteria are detected in the isolates which are Enterobacter spp., Citrobacter spp., Klebsiella spp., Staphylococcus aureus, Acinetobacter spp., and Bacillus spp. Bacterial identification by using 16s rDNA primer showed only Staphylococcus aureus was identified from the studied of bacterial isolates. Proper transportation, hygiene handling and proper storage are essential to prevent cross contamination of pathogenic bacteria in salad vegetables.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Nor, Izzafaiqaainaa
author_facet	Mohd Nor, Izzafaiqaainaa
author_sort	Mohd Nor, Izzafaiqaainaa
title	Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor
title_short	Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor
title_full	Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor
title_fullStr	Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor
title_full_unstemmed	Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor
title_sort	detection of pathogenic bacteria isolated from raw vegetables (ulam) / izzafaiqaainaa mohd nor
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50143/1/50143.pdf
_version_	1783734821961334784

Detection of pathogenic bacteria isolated from raw vegetables (ulam) / Izzafaiqaainaa Mohd Nor

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