Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid

Senna alata L. is commonly used as antimicrobial remedy against skin infection on human such as eczema, scabies, tinea infection, shingles and even herpes. Crude methanol extract of leaves from Senna alata were tested in vitro against three bacteria which are Escherichia coli (E.coli), Staphylococcu...

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Main Author:	Abdul Hamid, Nur Hazwani
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Antibiotic therapy Antibiotics Antibacterial agents
Online Access:	https://ir.uitm.edu.my/id/eprint/50231/1/50231.pdf
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id	my-uitm-ir.50231
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spelling	my-uitm-ir.502312021-09-09T06:47:01Z Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid 2017 Abdul Hamid, Nur Hazwani Antibiotic therapy. Antibiotics Antibacterial agents Flowers and flower culture. Ornamental plants Senna alata L. is commonly used as antimicrobial remedy against skin infection on human such as eczema, scabies, tinea infection, shingles and even herpes. Crude methanol extract of leaves from Senna alata were tested in vitro against three bacteria which are Escherichia coli (E.coli), Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis). This study was to determine antimicrobial susceptibility of crude methanol extract of Senna alata against E.coli, S. epidermidis and S. aureus in vitro and to identify the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) value of Senna alata methanol extract against E.coli, S. epidermidis and S. aureus in vitro. Disc diffusion method was used to determine the activity of selected bacteria against crude methanol extract of Senna alata. Minimal inhibitory concentration and minimal bactericidal concentration was used to identify lowest concentration that inhibit visible growth and killed most of the bacteria respectively. The highest zone of inhibition was observed against S. aureus (11.5mm) followed by S. epidermidis (11mm). However, the crude extract did not show any activity against E.coli. The value of MIC for S. aureus and S. epidermidis is 31.25 mg/ml. The value of MBC for both organisms S. aureus and S. epidermidis shows 500 mg/ml and 250 mg/ml respectively. This study showed that crude extract of Senna alata possessed antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis but not to E.coli. This finding provide new knowledge for treatment of common skin infection since the common bacterial skin infections are usually caused by Staphylococcus and Streptococcus species which is gram positive bacteria. However, further research about effect of Senna alata against wider range of bacteria and fungi and toxicological studies of the extract is recommended. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50231/ https://ir.uitm.edu.my/id/eprint/50231/1/50231.pdf text en public degree Universiti Teknologi Mara (Kampus Puncak Alam) Faculty of Health Sciences Wan Mohamad, Wan Kamil
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Wan Mohamad, Wan Kamil
topic	Antibiotic therapy Antibiotics Antibacterial agents Antibiotic therapy Antibiotics
spellingShingle	Antibiotic therapy Antibiotics Antibacterial agents Antibiotic therapy Antibiotics Abdul Hamid, Nur Hazwani Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid
description	Senna alata L. is commonly used as antimicrobial remedy against skin infection on human such as eczema, scabies, tinea infection, shingles and even herpes. Crude methanol extract of leaves from Senna alata were tested in vitro against three bacteria which are Escherichia coli (E.coli), Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis). This study was to determine antimicrobial susceptibility of crude methanol extract of Senna alata against E.coli, S. epidermidis and S. aureus in vitro and to identify the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) value of Senna alata methanol extract against E.coli, S. epidermidis and S. aureus in vitro. Disc diffusion method was used to determine the activity of selected bacteria against crude methanol extract of Senna alata. Minimal inhibitory concentration and minimal bactericidal concentration was used to identify lowest concentration that inhibit visible growth and killed most of the bacteria respectively. The highest zone of inhibition was observed against S. aureus (11.5mm) followed by S. epidermidis (11mm). However, the crude extract did not show any activity against E.coli. The value of MIC for S. aureus and S. epidermidis is 31.25 mg/ml. The value of MBC for both organisms S. aureus and S. epidermidis shows 500 mg/ml and 250 mg/ml respectively. This study showed that crude extract of Senna alata possessed antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis but not to E.coli. This finding provide new knowledge for treatment of common skin infection since the common bacterial skin infections are usually caused by Staphylococcus and Streptococcus species which is gram positive bacteria. However, further research about effect of Senna alata against wider range of bacteria and fungi and toxicological studies of the extract is recommended.
format	Thesis
qualification_level	Bachelor degree
author	Abdul Hamid, Nur Hazwani
author_facet	Abdul Hamid, Nur Hazwani
author_sort	Abdul Hamid, Nur Hazwani
title	Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid
title_short	Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid
title_full	Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid
title_fullStr	Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid
title_full_unstemmed	Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid
title_sort	determination of antibacterial activity of methanol extract of senna alata plant / nur hazwani abdul hamid
granting_institution	Universiti Teknologi Mara (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50231/1/50231.pdf
_version_	1783734827562827776

Determination of antibacterial activity of methanol extract of senna alata plant / Nur Hazwani Abdul Hamid

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