Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari

Staining of the nucleus is crucial in detecting the cancerous cells. In cytopathology, the nucleus was stained by hematoxylin which derived from logwood, Haematoxylum camphecianum. Bougainvillea Spectabilis (paper flower) and Dianthus Caryophyllus (carnation) have shown the staining ability towards...

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Main Author:	Sa'ari, Nur Syamimi
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QD Chemistry Chemicals
Online Access:	https://ir.uitm.edu.my/id/eprint/50736/1/50736.pdf
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spelling	my-uitm-ir.507362021-09-28T02:49:31Z Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari 2019-07 Sa'ari, Nur Syamimi QD Chemistry Flowers and flower culture. Ornamental plants Chemicals Staining of the nucleus is crucial in detecting the cancerous cells. In cytopathology, the nucleus was stained by hematoxylin which derived from logwood, Haematoxylum camphecianum. Bougainvillea Spectabilis (paper flower) and Dianthus Caryophyllus (carnation) have shown the staining ability towards the nucleus in the previous study. In order to apply a good fixation of the dye onto cells, a suitable mordant has to be selected. The different mordant used will give a different shade of color and color resistance to fading properties. Therefore, the aim of this study is to identify the most functional mordant between phosphotungstic acid and ferrous sulphate in color enhancement of Bougainvillea Spectabilis and Dianthus Caryophyllus. There were four parameters performed which were pH, concentration, qualitative microscopic evaluation and quantitative color intensity measurement (MIPAR software). Based on the result, ferrous sulphate enhance the staining quality better on Dianthus Caryophyllus than Bougainvillea Spectabilis with 4.4 pH, 16.75% concentration, Fair agreement and 163.35 pixels. Phosphotungstic acid mordant was not enhancing the staining quality from both Dianthus Caryophyllus and Bougainvillea Spectabilis dyes extract. All extracted dyes from both flowers without mordant and incorporated with phosphotungstic acid resulting in poor agreement in qualitative microscopic evaluation. Only Dianthus Caryophyllus incorporated with ferrous sulphate gave the highest agreement in qualitative microscopic evaluation. In conclusion, Dianthus Caryophyllus gave a good staining quality, although it is incomparable to control Papanicolaou (PAP) stain. Thus, further study of different types of mordant on Dianthus Caryophyllus and Bougainvillea Spectabilis dyes extract should be performed. 2019-07 Thesis https://ir.uitm.edu.my/id/eprint/50736/ https://ir.uitm.edu.my/id/eprint/50736/1/50736.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Abu, Mohd Nazri
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abu, Mohd Nazri
topic	QD Chemistry QD Chemistry Chemicals
spellingShingle	QD Chemistry QD Chemistry Chemicals Sa'ari, Nur Syamimi Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari
description	Staining of the nucleus is crucial in detecting the cancerous cells. In cytopathology, the nucleus was stained by hematoxylin which derived from logwood, Haematoxylum camphecianum. Bougainvillea Spectabilis (paper flower) and Dianthus Caryophyllus (carnation) have shown the staining ability towards the nucleus in the previous study. In order to apply a good fixation of the dye onto cells, a suitable mordant has to be selected. The different mordant used will give a different shade of color and color resistance to fading properties. Therefore, the aim of this study is to identify the most functional mordant between phosphotungstic acid and ferrous sulphate in color enhancement of Bougainvillea Spectabilis and Dianthus Caryophyllus. There were four parameters performed which were pH, concentration, qualitative microscopic evaluation and quantitative color intensity measurement (MIPAR software). Based on the result, ferrous sulphate enhance the staining quality better on Dianthus Caryophyllus than Bougainvillea Spectabilis with 4.4 pH, 16.75% concentration, Fair agreement and 163.35 pixels. Phosphotungstic acid mordant was not enhancing the staining quality from both Dianthus Caryophyllus and Bougainvillea Spectabilis dyes extract. All extracted dyes from both flowers without mordant and incorporated with phosphotungstic acid resulting in poor agreement in qualitative microscopic evaluation. Only Dianthus Caryophyllus incorporated with ferrous sulphate gave the highest agreement in qualitative microscopic evaluation. In conclusion, Dianthus Caryophyllus gave a good staining quality, although it is incomparable to control Papanicolaou (PAP) stain. Thus, further study of different types of mordant on Dianthus Caryophyllus and Bougainvillea Spectabilis dyes extract should be performed.
format	Thesis
qualification_level	Bachelor degree
author	Sa'ari, Nur Syamimi
author_facet	Sa'ari, Nur Syamimi
author_sort	Sa'ari, Nur Syamimi
title	Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari
title_short	Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari
title_full	Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari
title_fullStr	Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari
title_full_unstemmed	Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari
title_sort	comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / nur syamimi sa'ari
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50736/1/50736.pdf
_version_	1783734838245720064

Comparative study of ferrous sulphate and phosphotungstic acid mordants in enhancement of cytological natural dyes extracted from bougainvillae spectabilis and dianthus caryophyllus / Nur Syamimi Sa'ari

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