Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar.

In this study, krill protein hydrolysate was produced from krill (Euphausia pacifica) and its optimization effects on time and enzyme substrate ratio were determined. Krill protein was hydrolyzed by using Alcalase. Response surface methodology (RSM) was used to optimize the hydrolysis parameter. The...

Full description

Saved in:

Bibliographic Details
Main Author:	Jaafar, Naziera
Format:	Thesis
Language:	English
Published:	2011
Subjects:	Hydroxylation Physical and theoretical chemistry Analytical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/53060/1/53060.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.53060
record_format	uketd_dc
spelling	my-uitm-ir.530602021-12-14T09:23:32Z Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar. 2011 Jaafar, Naziera Hydroxylation Physical and theoretical chemistry Analytical chemistry In this study, krill protein hydrolysate was produced from krill (Euphausia pacifica) and its optimization effects on time and enzyme substrate ratio were determined. Krill protein was hydrolyzed by using Alcalase. Response surface methodology (RSM) was used to optimize the hydrolysis parameter. The parameters were time and enzyme substrate ratio with the percent nitrogen recovery (%NR) and degree of hydrolysis (%DH) being the responses. The levels of enzyme used were 1.38%, 2.00%, 3.50%, 5.00% and 5.62% while the time for hydrolysis taken were 31.72, 40.00, 60.00, 80.00 and 88.28 minutes. The optimum level for nitrogen recovery was 2.80% at 2.00% enzyme substrate ratio and 80 minutes of hydrolysis time with desirability of 0.74. For degree of hydrolysis, the optimum value was 4.04% at 4.07% enzyme substrate ratio for 44.03 minutes of hydrolysis time with the desirability 0.55. The pH and temperature were maintained constant throughout the experiment at pH 7.5 and 65°C respectively. 2011 Thesis https://ir.uitm.edu.my/id/eprint/53060/ https://ir.uitm.edu.my/id/eprint/53060/1/53060.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Normah (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Normah (Dr.)
topic	Hydroxylation Physical and theoretical chemistry Analytical chemistry
spellingShingle	Hydroxylation Physical and theoretical chemistry Analytical chemistry Jaafar, Naziera Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar.
description	In this study, krill protein hydrolysate was produced from krill (Euphausia pacifica) and its optimization effects on time and enzyme substrate ratio were determined. Krill protein was hydrolyzed by using Alcalase. Response surface methodology (RSM) was used to optimize the hydrolysis parameter. The parameters were time and enzyme substrate ratio with the percent nitrogen recovery (%NR) and degree of hydrolysis (%DH) being the responses. The levels of enzyme used were 1.38%, 2.00%, 3.50%, 5.00% and 5.62% while the time for hydrolysis taken were 31.72, 40.00, 60.00, 80.00 and 88.28 minutes. The optimum level for nitrogen recovery was 2.80% at 2.00% enzyme substrate ratio and 80 minutes of hydrolysis time with desirability of 0.74. For degree of hydrolysis, the optimum value was 4.04% at 4.07% enzyme substrate ratio for 44.03 minutes of hydrolysis time with the desirability 0.55. The pH and temperature were maintained constant throughout the experiment at pH 7.5 and 65°C respectively.
format	Thesis
qualification_level	Bachelor degree
author	Jaafar, Naziera
author_facet	Jaafar, Naziera
author_sort	Jaafar, Naziera
title	Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar.
title_short	Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar.
title_full	Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar.
title_fullStr	Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar.
title_full_unstemmed	Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar.
title_sort	optimization of krill (euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / naziera jaafar.
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2011
url	https://ir.uitm.edu.my/id/eprint/53060/1/53060.pdf
_version_	1783734859300077568

Optimization of krill (Euphausia pacifica) hydrolysate by alcalase (effect of time and enzyme substrate ratio) / Naziera Jaafar.

Similar Items