Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.

In this study, oriental angel wing {Pholas orientalis) hydrolysate was hydrolyzed by using Protamex. The total protein content in oriental angel wing per lOOg sample was 12%. Hydrolysis conditions were optimized by using response surface methodology (RSM). The model equations were proposed with rega...

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Main Author:	Mohd Bakri, Emilia Azrina
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Biochemistry Conditions and laws of chemical reactions
Online Access:	https://ir.uitm.edu.my/id/eprint/53083/1/53083.pdf
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id	my-uitm-ir.53083
record_format	uketd_dc
spelling	my-uitm-ir.530832021-12-15T04:26:12Z Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri. 2009 Mohd Bakri, Emilia Azrina Biochemistry Conditions and laws of chemical reactions In this study, oriental angel wing {Pholas orientalis) hydrolysate was hydrolyzed by using Protamex. The total protein content in oriental angel wing per lOOg sample was 12%. Hydrolysis conditions were optimized by using response surface methodology (RSM). The model equations were proposed with regard to the effects of time (min) and enzyme substrate ratio (%) on the degree of hydrolysis (DH) and nitrogen recovery (NR). By fitting the experimental data to the equation, the DH optimum levels for time (180min), ES (6%), %DH (5.11%) with desirability 0.596 at constant pH 7 and constant temperature 50°C were obtained. For %NR, the optimum levels for time (92.44 min), ES (3.21%), %NR (0.972%) with the desirability 0.935 were obtained. This study is important to any researcher to make a further analysis regarding the activity of enzyme hydrolysis especially when dealing with endoproteinase enzyme such as Protamex. This study also had open the opportunity for those who want to do further study on the sample namely oriental angel wing (Pholas orientalis) which is not yet popular among scientist. It also help to increase the economical support for those who supplied these bivalve species. 2009 Thesis https://ir.uitm.edu.my/id/eprint/53083/ https://ir.uitm.edu.my/id/eprint/53083/1/53083.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Normah (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Normah (Dr.)
topic	Biochemistry Conditions and laws of chemical reactions
spellingShingle	Biochemistry Conditions and laws of chemical reactions Mohd Bakri, Emilia Azrina Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
description	In this study, oriental angel wing {Pholas orientalis) hydrolysate was hydrolyzed by using Protamex. The total protein content in oriental angel wing per lOOg sample was 12%. Hydrolysis conditions were optimized by using response surface methodology (RSM). The model equations were proposed with regard to the effects of time (min) and enzyme substrate ratio (%) on the degree of hydrolysis (DH) and nitrogen recovery (NR). By fitting the experimental data to the equation, the DH optimum levels for time (180min), ES (6%), %DH (5.11%) with desirability 0.596 at constant pH 7 and constant temperature 50°C were obtained. For %NR, the optimum levels for time (92.44 min), ES (3.21%), %NR (0.972%) with the desirability 0.935 were obtained. This study is important to any researcher to make a further analysis regarding the activity of enzyme hydrolysis especially when dealing with endoproteinase enzyme such as Protamex. This study also had open the opportunity for those who want to do further study on the sample namely oriental angel wing (Pholas orientalis) which is not yet popular among scientist. It also help to increase the economical support for those who supplied these bivalve species.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Bakri, Emilia Azrina
author_facet	Mohd Bakri, Emilia Azrina
author_sort	Mohd Bakri, Emilia Azrina
title	Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_short	Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_full	Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_fullStr	Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_full_unstemmed	Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_sort	optimization of hydrolysis conditions for the production of oriental angel wing (pholas orientalis) hydrolysate by protamex: effect of time and enzyme substrate ratio / emilia azrina mohd bakri.
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/53083/1/53083.pdf
_version_	1783734861648887808

Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.

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