Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.

In this study, oriental angel wing {Pholas orientalis) hydrolysate was hydrolyzed by using Protamex. The total protein content in oriental angel wing per lOOg sample was 12%. Hydrolysis conditions were optimized by using response surface methodology (RSM). The model equations were proposed with rega...

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Main Author: Mohd Bakri, Emilia Azrina
Format: Thesis
Language:English
Published: 2009
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/53083/1/53083.pdf
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spelling my-uitm-ir.530832021-12-15T04:26:12Z Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri. 2009 Mohd Bakri, Emilia Azrina Biochemistry Conditions and laws of chemical reactions In this study, oriental angel wing {Pholas orientalis) hydrolysate was hydrolyzed by using Protamex. The total protein content in oriental angel wing per lOOg sample was 12%. Hydrolysis conditions were optimized by using response surface methodology (RSM). The model equations were proposed with regard to the effects of time (min) and enzyme substrate ratio (%) on the degree of hydrolysis (DH) and nitrogen recovery (NR). By fitting the experimental data to the equation, the DH optimum levels for time (180min), ES (6%), %DH (5.11%) with desirability 0.596 at constant pH 7 and constant temperature 50°C were obtained. For %NR, the optimum levels for time (92.44 min), ES (3.21%), %NR (0.972%) with the desirability 0.935 were obtained. This study is important to any researcher to make a further analysis regarding the activity of enzyme hydrolysis especially when dealing with endoproteinase enzyme such as Protamex. This study also had open the opportunity for those who want to do further study on the sample namely oriental angel wing (Pholas orientalis) which is not yet popular among scientist. It also help to increase the economical support for those who supplied these bivalve species. 2009 Thesis https://ir.uitm.edu.my/id/eprint/53083/ https://ir.uitm.edu.my/id/eprint/53083/1/53083.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Normah (Dr.)
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Ismail, Normah (Dr.)
topic Biochemistry
Conditions and laws of chemical reactions
spellingShingle Biochemistry
Conditions and laws of chemical reactions
Mohd Bakri, Emilia Azrina
Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
description In this study, oriental angel wing {Pholas orientalis) hydrolysate was hydrolyzed by using Protamex. The total protein content in oriental angel wing per lOOg sample was 12%. Hydrolysis conditions were optimized by using response surface methodology (RSM). The model equations were proposed with regard to the effects of time (min) and enzyme substrate ratio (%) on the degree of hydrolysis (DH) and nitrogen recovery (NR). By fitting the experimental data to the equation, the DH optimum levels for time (180min), ES (6%), %DH (5.11%) with desirability 0.596 at constant pH 7 and constant temperature 50°C were obtained. For %NR, the optimum levels for time (92.44 min), ES (3.21%), %NR (0.972%) with the desirability 0.935 were obtained. This study is important to any researcher to make a further analysis regarding the activity of enzyme hydrolysis especially when dealing with endoproteinase enzyme such as Protamex. This study also had open the opportunity for those who want to do further study on the sample namely oriental angel wing (Pholas orientalis) which is not yet popular among scientist. It also help to increase the economical support for those who supplied these bivalve species.
format Thesis
qualification_level Bachelor degree
author Mohd Bakri, Emilia Azrina
author_facet Mohd Bakri, Emilia Azrina
author_sort Mohd Bakri, Emilia Azrina
title Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_short Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_full Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_fullStr Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_full_unstemmed Optimization of hydrolysis conditions for the production of oriental angel wing (Pholas orientalis) hydrolysate by Protamex: effect of time and enzyme substrate ratio / Emilia Azrina Mohd Bakri.
title_sort optimization of hydrolysis conditions for the production of oriental angel wing (pholas orientalis) hydrolysate by protamex: effect of time and enzyme substrate ratio / emilia azrina mohd bakri.
granting_institution Universiti Teknologi MARA
granting_department Faculty of Applied Sciences
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/53083/1/53083.pdf
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