The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin

DNA is one of the most essential macromolecules in cells, holding the genetic information needed for living beings to grow and function. Finding a medium in which DNA is both soluble and stable over a long length of time is a tough task. As a result, deep eutectic solvents (DES) were proposed as a n...

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Main Author:	Ariffin, Nur Fikriyah
Format:	Thesis
Language:	English
Published:	2022
Subjects:	DNA Deoxyribonucleic acids Acids and bases
Online Access:	https://ir.uitm.edu.my/id/eprint/59907/1/59907.pdf
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id	my-uitm-ir.59907
record_format	uketd_dc
spelling	my-uitm-ir.599072022-11-02T03:49:39Z The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin 2022-10-27 Ariffin, Nur Fikriyah DNA. Deoxyribonucleic acids Acids and bases DNA is one of the most essential macromolecules in cells, holding the genetic information needed for living beings to grow and function. Finding a medium in which DNA is both soluble and stable over a long length of time is a tough task. As a result, deep eutectic solvents (DES) were proposed as a novel solvent for this study to replace water or aqueous solutions. The purpose of this research is to identify effect of different concentration of DES on DNA as storage medium using a UV- Visible Spectrophotometer. DES was prepared by heating malonic acid and choline chloride at ratio 1:1 and 80 °C until a clear liquid emerged. Following that, a little amount of calf thymus DNA was dissolved and homogeneously mixed in deionised water in an ice bath (4 °C) for 24 hours. For UV-Vis, 100 μm DNA was produced and combined with various concentrations of hydrated DES: 25%, 50%, and 75%. The pure DNA concentration was 0.0875846 M. The purity of DNA is 1.89 using the A260/A280 calculation. 25% displays the closest peak with control, a concentration of 25% was determined to preserve the stability of DNA. FTIR analysis revealed a few functional groups in pure DNA, pure DES solution and DNA in DES solution. 2022-10 Thesis https://ir.uitm.edu.my/id/eprint/59907/ https://ir.uitm.edu.my/id/eprint/59907/1/59907.pdf text en public degree Universiti Teknologi Mara Perlis Faculty of Applied Science
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	DNA Deoxyribonucleic acids Acids and bases
spellingShingle	DNA Deoxyribonucleic acids Acids and bases Ariffin, Nur Fikriyah The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin
description	DNA is one of the most essential macromolecules in cells, holding the genetic information needed for living beings to grow and function. Finding a medium in which DNA is both soluble and stable over a long length of time is a tough task. As a result, deep eutectic solvents (DES) were proposed as a novel solvent for this study to replace water or aqueous solutions. The purpose of this research is to identify effect of different concentration of DES on DNA as storage medium using a UV- Visible Spectrophotometer. DES was prepared by heating malonic acid and choline chloride at ratio 1:1 and 80 °C until a clear liquid emerged. Following that, a little amount of calf thymus DNA was dissolved and homogeneously mixed in deionised water in an ice bath (4 °C) for 24 hours. For UV-Vis, 100 μm DNA was produced and combined with various concentrations of hydrated DES: 25%, 50%, and 75%. The pure DNA concentration was 0.0875846 M. The purity of DNA is 1.89 using the A260/A280 calculation. 25% displays the closest peak with control, a concentration of 25% was determined to preserve the stability of DNA. FTIR analysis revealed a few functional groups in pure DNA, pure DES solution and DNA in DES solution.
format	Thesis
qualification_level	Bachelor degree
author	Ariffin, Nur Fikriyah
author_facet	Ariffin, Nur Fikriyah
author_sort	Ariffin, Nur Fikriyah
title	The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin
title_short	The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin
title_full	The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin
title_fullStr	The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin
title_full_unstemmed	The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin
title_sort	stability of dna in chloride-malonic acid deep eutectic solvent at different concentration / nur fikriyah ariffin
granting_institution	Universiti Teknologi Mara Perlis
granting_department	Faculty of Applied Science
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/59907/1/59907.pdf
_version_	1783735067187609600

The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin

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