Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari

Osteosarcoma is a very aggressive malignancy of the bone that usually at times of diagnosis, patients have already presented with metastasis. In this study, we tried to detect the cellular adhesion molecules (VCAM-1 and ICAM-3) that are believed to be involved in tumor cells migration that might cau...

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Main Author:	Zahari, Khalilah Mastura
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Neoplasms Tumors Oncology
Online Access:	https://ir.uitm.edu.my/id/eprint/72163/1/72163.pdf
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id	my-uitm-ir.72163
record_format	uketd_dc
spelling	my-uitm-ir.721632024-01-02T07:42:31Z Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari 2012 Zahari, Khalilah Mastura Neoplasms. Tumors. Oncology Osteosarcoma is a very aggressive malignancy of the bone that usually at times of diagnosis, patients have already presented with metastasis. In this study, we tried to detect the cellular adhesion molecules (VCAM-1 and ICAM-3) that are believed to be involved in tumor cells migration that might cause the metastasis. Two osteosarcoma samples were harvested from two patients before and after chemotherapy respectively. The tissue sections were stained with Hematoxylin and Eosin (H&E) staining for reviews and subsequently stained for VCAM-1 and ICAM-3 antibody by Immunohistochemical (IHC) staining to observe these cellular adhesion molecules expressions. At the same time, the area and number of nuclei were measured and compared before and after chemotherapy to relate it with the expression of VCAM-1 and ICAM-3, also, to relate it with the current clinical situation of the patients. In this study, no significant expression of ICAM-3 was observed in both Patient 1 and Patient 2. However, there was a strong VCAM-1 expression and also an increased of number and area of tumor cells in Patient 2 that was presented with early local recurrence. This could be associated with the poor response to chemotherapy and also could be a potential prognostic marker in patients with osteosarcoma. 2012 Thesis https://ir.uitm.edu.my/id/eprint/72163/ https://ir.uitm.edu.my/id/eprint/72163/1/72163.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Medicine Ab. Rahim, Sharaniza
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ab. Rahim, Sharaniza
topic	Neoplasms Tumors Oncology
spellingShingle	Neoplasms Tumors Oncology Zahari, Khalilah Mastura Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari
description	Osteosarcoma is a very aggressive malignancy of the bone that usually at times of diagnosis, patients have already presented with metastasis. In this study, we tried to detect the cellular adhesion molecules (VCAM-1 and ICAM-3) that are believed to be involved in tumor cells migration that might cause the metastasis. Two osteosarcoma samples were harvested from two patients before and after chemotherapy respectively. The tissue sections were stained with Hematoxylin and Eosin (H&E) staining for reviews and subsequently stained for VCAM-1 and ICAM-3 antibody by Immunohistochemical (IHC) staining to observe these cellular adhesion molecules expressions. At the same time, the area and number of nuclei were measured and compared before and after chemotherapy to relate it with the expression of VCAM-1 and ICAM-3, also, to relate it with the current clinical situation of the patients. In this study, no significant expression of ICAM-3 was observed in both Patient 1 and Patient 2. However, there was a strong VCAM-1 expression and also an increased of number and area of tumor cells in Patient 2 that was presented with early local recurrence. This could be associated with the poor response to chemotherapy and also could be a potential prognostic marker in patients with osteosarcoma.
format	Thesis
qualification_level	Bachelor degree
author	Zahari, Khalilah Mastura
author_facet	Zahari, Khalilah Mastura
author_sort	Zahari, Khalilah Mastura
title	Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari
title_short	Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari
title_full	Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari
title_fullStr	Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari
title_full_unstemmed	Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari
title_sort	preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / khalilah mastura zahari
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Medicine
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/72163/1/72163.pdf
_version_	1794191885470793728

Preliminary study of osteosarcoma tissue proteins of pre and post-chemotherapy patient / Khalilah Mastura Zahari

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