The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi

Oxidative stress induced by the aging process was known to cause impairment in female reproductive and antioxidant defense system. Tocotrienol (TCT), through its antioxidant properties has been suggested to play a role in reducing oxidative stress and promoting fertility. The present study evaluates...

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Main Author:	Mohamed Almi, Nur Aida Dalilah
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QL Zoology
Online Access:	https://ir.uitm.edu.my/id/eprint/72181/1/72181.pdf
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id	my-uitm-ir.72181
record_format	uketd_dc
spelling	my-uitm-ir.721812024-01-03T08:13:40Z The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi 2013 Mohamed Almi, Nur Aida Dalilah QL Zoology Tocotrienol. Tocopherol. Vitamin E Oxidative stress induced by the aging process was known to cause impairment in female reproductive and antioxidant defense system. Tocotrienol (TCT), through its antioxidant properties has been suggested to play a role in reducing oxidative stress and promoting fertility. The present study evaluates the protective effect of TCT on the ovarian function which was assessed from the response for ovulation process, the quality and developmental competence of embryos produced, and the activity of catalase (CAT), a component of antioxidant defense system. Twelve weeks old female mice were subjected to daily oral gavages of either corn oil (vehicle) or TCT at different doses of 60, 120 and 240 mg kg _1 body weight (BW) for 8 weeks duration. Age control groups consisted of young mice (7 weeks old) and aging mice (20 weeks old). Animals were then, superovulated and cohabited with a fertile male at a ratio of 1:1. After 48 hours, animals were euthanized, the fallopian tubes were excised and embryos were flushed out. The embryos recovered were then be counted and graded based on its morphology. Only normal embryos were cultured to observe the developmental competence from 2 cells to expanded blastocyst stage. Counting the total number of embryos recovered quantified the ovarian response for ovulation process whereby grading the embryo morphology determined the embryo quality. We found out that the age related decline of embryo count, quality and in vitro development from 2 cells to 4 cells stage was improved with TCT treatment at the dose of 120 and 240 mg kg'1 BW. The embryos recovered from 240 mg kg"1 BW TCT treatment group also denoted to have a higher progression rate at 4 cells to morula stage when compared with all groups. There is no significant difference in CAT level in plasma and ovarian tissue samples in all TCT treatment groups as compared to aging control. Therefore, TCT is found to have a beneficial effect on the ovulation response, embryonic quality and in vitro development from 2 cells to morula stage in aging mice. 2013 Thesis https://ir.uitm.edu.my/id/eprint/72181/ https://ir.uitm.edu.my/id/eprint/72181/1/72181.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Medicine Azme, Nasibah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Azme, Nasibah
topic	QL Zoology QL Zoology
spellingShingle	QL Zoology QL Zoology Mohamed Almi, Nur Aida Dalilah The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
description	Oxidative stress induced by the aging process was known to cause impairment in female reproductive and antioxidant defense system. Tocotrienol (TCT), through its antioxidant properties has been suggested to play a role in reducing oxidative stress and promoting fertility. The present study evaluates the protective effect of TCT on the ovarian function which was assessed from the response for ovulation process, the quality and developmental competence of embryos produced, and the activity of catalase (CAT), a component of antioxidant defense system. Twelve weeks old female mice were subjected to daily oral gavages of either corn oil (vehicle) or TCT at different doses of 60, 120 and 240 mg kg _1 body weight (BW) for 8 weeks duration. Age control groups consisted of young mice (7 weeks old) and aging mice (20 weeks old). Animals were then, superovulated and cohabited with a fertile male at a ratio of 1:1. After 48 hours, animals were euthanized, the fallopian tubes were excised and embryos were flushed out. The embryos recovered were then be counted and graded based on its morphology. Only normal embryos were cultured to observe the developmental competence from 2 cells to expanded blastocyst stage. Counting the total number of embryos recovered quantified the ovarian response for ovulation process whereby grading the embryo morphology determined the embryo quality. We found out that the age related decline of embryo count, quality and in vitro development from 2 cells to 4 cells stage was improved with TCT treatment at the dose of 120 and 240 mg kg'1 BW. The embryos recovered from 240 mg kg"1 BW TCT treatment group also denoted to have a higher progression rate at 4 cells to morula stage when compared with all groups. There is no significant difference in CAT level in plasma and ovarian tissue samples in all TCT treatment groups as compared to aging control. Therefore, TCT is found to have a beneficial effect on the ovulation response, embryonic quality and in vitro development from 2 cells to morula stage in aging mice.
format	Thesis
qualification_level	Bachelor degree
author	Mohamed Almi, Nur Aida Dalilah
author_facet	Mohamed Almi, Nur Aida Dalilah
author_sort	Mohamed Almi, Nur Aida Dalilah
title	The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_short	The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_full	The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_fullStr	The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_full_unstemmed	The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_sort	preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / nur aida dalilah mohamed almi
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Medicine
publishDate	2013
url	https://ir.uitm.edu.my/id/eprint/72181/1/72181.pdf
_version_	1794191889918853120

The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi

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