Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man

Food poisoning and foodborne illnesses caused by microbial deterioration during preparation and storage are becoming more common across the world. Synthetic additives and antimicrobial agents are used in food processing to alleviate the development of bacteria, minimizing contamination and extending...

Full description

Saved in:

Bibliographic Details
Main Author:	Che Man, Che Amira Izzati
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QR Microbiology
Online Access:	https://ir.uitm.edu.my/id/eprint/76717/2/76717.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.76717
record_format	uketd_dc
spelling	my-uitm-ir.767172024-02-22T09:03:33Z Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man 2022 Che Man, Che Amira Izzati QR Microbiology Food poisoning and foodborne illnesses caused by microbial deterioration during preparation and storage are becoming more common across the world. Synthetic additives and antimicrobial agents are used in food processing to alleviate the development of bacteria, minimizing contamination and extending food shelf life. Unfortunately, synthetic chemical additives have harmful effects and might lead to respiratory or other health issues. Due to increasing attention to that, there is a greater need for natural inhibitors as alternatives to synthetic antimicrobial agent. Efficacy of Swietenia macrophylla has been investigated in many studies, however its antibiofilm and antibacterial against foodborne pathogens remains not well investigated. The present work was performed to examine the phytochemicals constituents, antibacterial and antibiofilm activities of S. macrophylla leaves ethanolic extract against S. typhimurium ATCC 14028, S. sonnei ATCC 33862, E. coli ATCC 25922 and P. aeruginosa ATCC 10145. Antibiofilm activities of S. macrophylla was studied using the crystal violet assay, time killing assay and biofilm inhibitory concentration. Antibacterial activities were done by the determination of minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). Gas Chromatography-Mass Spectrometry (GC-MS) was employed to analyze the phytochemical compounds of the plant. GC-MS of S. macrophylla plant leaves extract, revealed the existence of Germanicol (11.50%), 3,4-Altrosan (1.44%), 2,4-Di-tert butylphenol (0.49%) and n-Hexadecanoic (0.23%). The ethanolic extract of S. macrophylla had the lowest MIC values against P 2022 Thesis https://ir.uitm.edu.my/id/eprint/76717/ https://ir.uitm.edu.my/id/eprint/76717/2/76717.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Raja Yahya, Mohd Fakharul Zaman
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Raja Yahya, Mohd Fakharul Zaman
topic	QR Microbiology
spellingShingle	QR Microbiology Che Man, Che Amira Izzati Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man
description	Food poisoning and foodborne illnesses caused by microbial deterioration during preparation and storage are becoming more common across the world. Synthetic additives and antimicrobial agents are used in food processing to alleviate the development of bacteria, minimizing contamination and extending food shelf life. Unfortunately, synthetic chemical additives have harmful effects and might lead to respiratory or other health issues. Due to increasing attention to that, there is a greater need for natural inhibitors as alternatives to synthetic antimicrobial agent. Efficacy of Swietenia macrophylla has been investigated in many studies, however its antibiofilm and antibacterial against foodborne pathogens remains not well investigated. The present work was performed to examine the phytochemicals constituents, antibacterial and antibiofilm activities of S. macrophylla leaves ethanolic extract against S. typhimurium ATCC 14028, S. sonnei ATCC 33862, E. coli ATCC 25922 and P. aeruginosa ATCC 10145. Antibiofilm activities of S. macrophylla was studied using the crystal violet assay, time killing assay and biofilm inhibitory concentration. Antibacterial activities were done by the determination of minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). Gas Chromatography-Mass Spectrometry (GC-MS) was employed to analyze the phytochemical compounds of the plant. GC-MS of S. macrophylla plant leaves extract, revealed the existence of Germanicol (11.50%), 3,4-Altrosan (1.44%), 2,4-Di-tert butylphenol (0.49%) and n-Hexadecanoic (0.23%). The ethanolic extract of S. macrophylla had the lowest MIC values against P
format	Thesis
qualification_level	Master's degree
author	Che Man, Che Amira Izzati
author_facet	Che Man, Che Amira Izzati
author_sort	Che Man, Che Amira Izzati
title	Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man
title_short	Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man
title_full	Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man
title_fullStr	Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man
title_full_unstemmed	Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man
title_sort	antibiofilm and antibacterial activities of ethanolic extract of swietenia macrophylla king against foodborne pathogens / che amira izzati che man
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/76717/2/76717.pdf
_version_	1794191909129814016

Antibiofilm and antibacterial activities of ethanolic extract of Swietenia macrophylla king against foodborne pathogens / Che Amira Izzati Che Man

Similar Items