Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman

Candida albicans is a microbial fungus that exists as a commensal member of the human microbiome and an opportunistic pathogen. Biofilm formation by this fungal pathogen occurs mostly in the mucosa or endothelium associated with candidiasis and also colonizes medical devices, such as vascular and ur...

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Main Author:	Kamaruzzaman, Alya Nur Athirah
Format:	Thesis
Language:	English
Published:	2022
Subjects:	Molding (Chemical technology)
Online Access:	https://ir.uitm.edu.my/id/eprint/77901/1/77901.pdf
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id	my-uitm-ir.77901
record_format	uketd_dc
spelling	my-uitm-ir.779012023-05-29T07:11:20Z Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman 2022 Kamaruzzaman, Alya Nur Athirah Molding (Chemical technology) Candida albicans is a microbial fungus that exists as a commensal member of the human microbiome and an opportunistic pathogen. Biofilm formation by this fungal pathogen occurs mostly in the mucosa or endothelium associated with candidiasis and also colonizes medical devices, such as vascular and urinary catheters and dentures. Improper use of antifungal agents has caused drug resistance. It happened when there are too many antifungal drugs used to treat diseases caused by C. albicans. The aim of this research was to determine the effects of the antifungal creams on biomass, viability and biochemical composition of C. albicans biofilm. Four commercial antifungal creams were selected herein namely econazole nitrate (Cream A), miconazole nitrate (Cream B), ketoconazole (Cream C) and tolnaftate (Cream D) -based antifungal creams. Crystal violet assay, resazurin assay and infrared spectral analysis were performed to determine the biomass, viability and biochemical composition of C. albicans biofilm respectively. Among these four antifungal creams, miconazole nitrate-based antifungal cream (Cream B) showed the strongest inhibition of biofilm biomass (65.12%) and viability (17.08%). Miconazole nitrate-based antifungal cream showed that nucleic acid was affected through the shifting of Fourier transform infrared (FTIR) spectra at a wavenumber of 1240 cm-1. The findings from the present study may be useful to control disease caused by C. albicans such as candisiasis which had been categorised as a main problem affected on human being. 2022 Thesis https://ir.uitm.edu.my/id/eprint/77901/ https://ir.uitm.edu.my/id/eprint/77901/1/77901.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Science Raja Yahya, Mohd Fakharul Zaman
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Raja Yahya, Mohd Fakharul Zaman
topic	Molding (Chemical technology)
spellingShingle	Molding (Chemical technology) Kamaruzzaman, Alya Nur Athirah Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman
description	Candida albicans is a microbial fungus that exists as a commensal member of the human microbiome and an opportunistic pathogen. Biofilm formation by this fungal pathogen occurs mostly in the mucosa or endothelium associated with candidiasis and also colonizes medical devices, such as vascular and urinary catheters and dentures. Improper use of antifungal agents has caused drug resistance. It happened when there are too many antifungal drugs used to treat diseases caused by C. albicans. The aim of this research was to determine the effects of the antifungal creams on biomass, viability and biochemical composition of C. albicans biofilm. Four commercial antifungal creams were selected herein namely econazole nitrate (Cream A), miconazole nitrate (Cream B), ketoconazole (Cream C) and tolnaftate (Cream D) -based antifungal creams. Crystal violet assay, resazurin assay and infrared spectral analysis were performed to determine the biomass, viability and biochemical composition of C. albicans biofilm respectively. Among these four antifungal creams, miconazole nitrate-based antifungal cream (Cream B) showed the strongest inhibition of biofilm biomass (65.12%) and viability (17.08%). Miconazole nitrate-based antifungal cream showed that nucleic acid was affected through the shifting of Fourier transform infrared (FTIR) spectra at a wavenumber of 1240 cm-1. The findings from the present study may be useful to control disease caused by C. albicans such as candisiasis which had been categorised as a main problem affected on human being.
format	Thesis
qualification_level	Master's degree
author	Kamaruzzaman, Alya Nur Athirah
author_facet	Kamaruzzaman, Alya Nur Athirah
author_sort	Kamaruzzaman, Alya Nur Athirah
title	Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman
title_short	Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman
title_full	Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman
title_fullStr	Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman
title_full_unstemmed	Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman
title_sort	inhibition of candida albicans biofilm using topical antifungal creams / alya nur athirah kamaruzzaman
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Science
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/77901/1/77901.pdf
_version_	1783736178176950272

Inhibition of candida albicans biofilm using topical antifungal creams / Alya Nur Athirah Kamaruzzaman

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