Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz

Phytochemical investigation on the bark of Malaysian Croton laevifolius Blume (Euphorbiaceae) was carried out with an intention of isolating and identifying its chemical constituents. The bark was successively extracted with the non polar (hexane), medium polar (dichloromethane) and polar (methanol)...

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Main Author:	Aziz, Ahmad Nazif
Format:	Thesis
Language:	English
Published:	2018
Subjects:	QR Microbiology
Online Access:	https://ir.uitm.edu.my/id/eprint/83052/1/83052.pdf
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spelling	my-uitm-ir.830522024-03-14T02:43:34Z Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz 2018 Aziz, Ahmad Nazif QR Microbiology Phytochemical investigation on the bark of Malaysian Croton laevifolius Blume (Euphorbiaceae) was carried out with an intention of isolating and identifying its chemical constituents. The bark was successively extracted with the non polar (hexane), medium polar (dichloromethane) and polar (methanol) organic solvents. These extracts were evaluated for cytotoxicity, anti-inflammatory and antidiabetic activities. The hexane extract showed potential cytotoxicity against MCF-7 cell line and mild cytotoxicity against A549, WRL-68, PC-3 and A375 cells while dichloromethane (DCM) extract indicated mild and selective activity against A549 and A375 only. The hexane and DCM extracts were subjected to isolation and purification using various chromatographic techniques such as Medium Pressure Liquid Chromatography (MPLC), Radial Chromatography (RC) and Recycling Preparative High Performance Liquid Chromatography (RHPLC). Structures of the chemical compounds were elucidated using various spectroscopic techniques such as UV, IR, ID-NMR ('H, 13C, DEPT and APT), 2D-NMR (COSY, HSQC, HMBC and NOESY) and mass spectrometry. This study has led to the isolation of fourteen compounds, in which seven new and one known clerodane type diterpene named crovatin, as well as one eudesmane-type sesquiterpene named crytomeridiol were isolated from the DCM extract. This is the first occurrence of cryptomeridiol in Croton species. The new diterpenes were deduced as laevifin A, laevifin B, laevifin C, laevifin D, laevifin E, laevifin F and laevifin G. Subsequently, the hexane extract yielded laevifin B, three oleanane triterpenes: /J-amyrin, /3-amyrone and acetyl aleuritolic acid; one steroid (/3-sitostenone) and one flavonoid named pachypodol. 2018 Thesis https://ir.uitm.edu.my/id/eprint/83052/ https://ir.uitm.edu.my/id/eprint/83052/1/83052.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Ismail, Nor Hadiani Awang, Khalijah Ahmat, Norizan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Nor Hadiani Awang, Khalijah Ahmat, Norizan
topic	QR Microbiology
spellingShingle	QR Microbiology Aziz, Ahmad Nazif Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz
description	Phytochemical investigation on the bark of Malaysian Croton laevifolius Blume (Euphorbiaceae) was carried out with an intention of isolating and identifying its chemical constituents. The bark was successively extracted with the non polar (hexane), medium polar (dichloromethane) and polar (methanol) organic solvents. These extracts were evaluated for cytotoxicity, anti-inflammatory and antidiabetic activities. The hexane extract showed potential cytotoxicity against MCF-7 cell line and mild cytotoxicity against A549, WRL-68, PC-3 and A375 cells while dichloromethane (DCM) extract indicated mild and selective activity against A549 and A375 only. The hexane and DCM extracts were subjected to isolation and purification using various chromatographic techniques such as Medium Pressure Liquid Chromatography (MPLC), Radial Chromatography (RC) and Recycling Preparative High Performance Liquid Chromatography (RHPLC). Structures of the chemical compounds were elucidated using various spectroscopic techniques such as UV, IR, ID-NMR ('H, 13C, DEPT and APT), 2D-NMR (COSY, HSQC, HMBC and NOESY) and mass spectrometry. This study has led to the isolation of fourteen compounds, in which seven new and one known clerodane type diterpene named crovatin, as well as one eudesmane-type sesquiterpene named crytomeridiol were isolated from the DCM extract. This is the first occurrence of cryptomeridiol in Croton species. The new diterpenes were deduced as laevifin A, laevifin B, laevifin C, laevifin D, laevifin E, laevifin F and laevifin G. Subsequently, the hexane extract yielded laevifin B, three oleanane triterpenes: /J-amyrin, /3-amyrone and acetyl aleuritolic acid; one steroid (/3-sitostenone) and one flavonoid named pachypodol.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Aziz, Ahmad Nazif
author_facet	Aziz, Ahmad Nazif
author_sort	Aziz, Ahmad Nazif
title	Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz
title_short	Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz
title_full	Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz
title_fullStr	Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz
title_full_unstemmed	Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz
title_sort	chemical constituents of croton laevifolius blume bark / ahmad nazif aziz
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2018
url	https://ir.uitm.edu.my/id/eprint/83052/1/83052.pdf
_version_	1794191964281765888

Chemical constituents of croton laevifolius blume bark / Ahmad Nazif Aziz

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