In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir

Catharanthus roseus has high significant study due to its medicinal properties, but there is still lacks of information about the best combination of the hormones of the root induction. Thus, C. roseus are propagated by using plantlets through the techniques of tissue culture. The objectives of the...

Full description

Saved in:

Bibliographic Details
Main Author:	Mohamed Nazir, Siti Nazliah
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Medicinal plants (Culture only)
Online Access:	https://ir.uitm.edu.my/id/eprint/83852/1/83852.PDF
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.83852
record_format	uketd_dc
spelling	my-uitm-ir.838522024-01-22T02:24:42Z In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir 2016 Mohamed Nazir, Siti Nazliah Medicinal plants (Culture only) Catharanthus roseus has high significant study due to its medicinal properties, but there is still lacks of information about the best combination of the hormones of the root induction. Thus, C. roseus are propagated by using plantlets through the techniques of tissue culture. The objectives of the study are to investigate the cells formation by subculturing of C. roseus in MS media and to evaluate the performance of C. roseus plantlets by using different concentration of the hormones. The subject was treated with control (C) and four different treatments that consist of different combination of hormones of auxin and cytokinin (T1, T2, T3 and T4). Begins with the preparation of the media, Murashige and Skoog (MS) media was used. The plantlets then were transferred into the media to be incubate at 25°C for 8 weeks with the photoperiod of 16:8 hours. In week 4, the white callus shows friable structure compared to the green callus that is more compact and granular in all treatments. The cross section of the stem exhibit a ring structure of discrete vascular bundle with a narrow cortex situated externally indicates a dicots plants. The leaves of the control environment is generally yellow in color while greenish color observed in the T1 to T4 treatments, especially in T4. In week 8, the roots induction proliferates more in the T4. While the concentration of cytokinin was held constant at 0.5 mg/ml, Tl with equal concentration of auxin-cytokinin mostly show the growth of shoots. T2, T3 and T4 with concentration auxin of 1.0 mg/ml, 1.5 mg/ml, 2.0 mg/ml respectively show the growth of roots. However, the most number of generated roots come from the T4. 2016 Thesis https://ir.uitm.edu.my/id/eprint/83852/ https://ir.uitm.edu.my/id/eprint/83852/1/83852.PDF text en public degree Universiti Teknologi MARA Pahang Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Medicinal plants (Culture only)
spellingShingle	Medicinal plants (Culture only) Mohamed Nazir, Siti Nazliah In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
description	Catharanthus roseus has high significant study due to its medicinal properties, but there is still lacks of information about the best combination of the hormones of the root induction. Thus, C. roseus are propagated by using plantlets through the techniques of tissue culture. The objectives of the study are to investigate the cells formation by subculturing of C. roseus in MS media and to evaluate the performance of C. roseus plantlets by using different concentration of the hormones. The subject was treated with control (C) and four different treatments that consist of different combination of hormones of auxin and cytokinin (T1, T2, T3 and T4). Begins with the preparation of the media, Murashige and Skoog (MS) media was used. The plantlets then were transferred into the media to be incubate at 25°C for 8 weeks with the photoperiod of 16:8 hours. In week 4, the white callus shows friable structure compared to the green callus that is more compact and granular in all treatments. The cross section of the stem exhibit a ring structure of discrete vascular bundle with a narrow cortex situated externally indicates a dicots plants. The leaves of the control environment is generally yellow in color while greenish color observed in the T1 to T4 treatments, especially in T4. In week 8, the roots induction proliferates more in the T4. While the concentration of cytokinin was held constant at 0.5 mg/ml, Tl with equal concentration of auxin-cytokinin mostly show the growth of shoots. T2, T3 and T4 with concentration auxin of 1.0 mg/ml, 1.5 mg/ml, 2.0 mg/ml respectively show the growth of roots. However, the most number of generated roots come from the T4.
format	Thesis
qualification_level	Bachelor degree
author	Mohamed Nazir, Siti Nazliah
author_facet	Mohamed Nazir, Siti Nazliah
author_sort	Mohamed Nazir, Siti Nazliah
title	In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_short	In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_full	In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_fullStr	In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_full_unstemmed	In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_sort	in vitro root induction of catharanthus roseus / siti nazliah mohamed nazir
granting_institution	Universiti Teknologi MARA Pahang
granting_department	Faculty of Applied Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/83852/1/83852.PDF
_version_	1794191976015331328

In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir

Similar Items