Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi

Butt rot disease caused by Thielaviopsis paradoxa is an important disease of pineapple planting material in Malaysia. Plant extracts and antagonistic bacteria are potential biocontrol agents as they are effective and safer than chemical controls. In this study, five groups of antagonistic bacteria w...

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Main Author:	Mat Nawi, Nurnadirah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	QR Microbiology
Online Access:	https://ir.uitm.edu.my/id/eprint/84275/1/84275.pdf
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spelling	my-uitm-ir.842752024-03-14T02:43:31Z Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi 2018 Mat Nawi, Nurnadirah QR Microbiology Butt rot disease caused by Thielaviopsis paradoxa is an important disease of pineapple planting material in Malaysia. Plant extracts and antagonistic bacteria are potential biocontrol agents as they are effective and safer than chemical controls. In this study, five groups of antagonistic bacteria were isolated from soil sampled from pineapple plants infected with butt rot disease and non-infected plants and four species of plant extracts, namely Leucaena leucocephala (leaf, bark) Eucalyptus camaldulensis (leaf), Alpinia galanga (rhizome) and Cinnamomum iners (bark) were tested against fungal pathogen. In vitro antifungal potential of both biocontrol agents were evaluated against T. paradoxa. Well diffusion method was used to evaluate antifungal activity of plant extracts, while dual culture method was used to test the antifungal activity of antagonistic bacteria. The result indicated that, among all the tested extracts, three ethanol extracts of L. leucocephala bark, E. camaldulensis leaf and A. galangdl rhizome and two chloroform extracts of L. leucocephala leaf and C. iners bark have showed their antifungal activities with 1.25%, 1.5%, 1.5%, 2.25% and 42.75% radial growth inhibition. Antagonistic bacteria, Bl showed the highest radial growth inhibition with 75%. Results also showed that B2, B3, B4 and B5 bacteria exhibited positive inhibition towards the pathogen with 70.75%, 57.25%, 56.25% and 47.50% radial growth inhibition, respectively. Microscopic observation of antifungal activities by plant extracts and antagonistic bacteria showed that the antifungal activities were via degradation, retardation, malformation, swelling, coagulation and breakage of the hyphae of T. paradoxa 2018 Thesis https://ir.uitm.edu.my/id/eprint/84275/ https://ir.uitm.edu.my/id/eprint/84275/1/84275.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology Che Mohd Ramli, Neni Kartini
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Che Mohd Ramli, Neni Kartini
topic	QR Microbiology
spellingShingle	QR Microbiology Mat Nawi, Nurnadirah Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi
description	Butt rot disease caused by Thielaviopsis paradoxa is an important disease of pineapple planting material in Malaysia. Plant extracts and antagonistic bacteria are potential biocontrol agents as they are effective and safer than chemical controls. In this study, five groups of antagonistic bacteria were isolated from soil sampled from pineapple plants infected with butt rot disease and non-infected plants and four species of plant extracts, namely Leucaena leucocephala (leaf, bark) Eucalyptus camaldulensis (leaf), Alpinia galanga (rhizome) and Cinnamomum iners (bark) were tested against fungal pathogen. In vitro antifungal potential of both biocontrol agents were evaluated against T. paradoxa. Well diffusion method was used to evaluate antifungal activity of plant extracts, while dual culture method was used to test the antifungal activity of antagonistic bacteria. The result indicated that, among all the tested extracts, three ethanol extracts of L. leucocephala bark, E. camaldulensis leaf and A. galangdl rhizome and two chloroform extracts of L. leucocephala leaf and C. iners bark have showed their antifungal activities with 1.25%, 1.5%, 1.5%, 2.25% and 42.75% radial growth inhibition. Antagonistic bacteria, Bl showed the highest radial growth inhibition with 75%. Results also showed that B2, B3, B4 and B5 bacteria exhibited positive inhibition towards the pathogen with 70.75%, 57.25%, 56.25% and 47.50% radial growth inhibition, respectively. Microscopic observation of antifungal activities by plant extracts and antagonistic bacteria showed that the antifungal activities were via degradation, retardation, malformation, swelling, coagulation and breakage of the hyphae of T. paradoxa
format	Thesis
qualification_level	Master's degree
author	Mat Nawi, Nurnadirah
author_facet	Mat Nawi, Nurnadirah
author_sort	Mat Nawi, Nurnadirah
title	Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi
title_short	Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi
title_full	Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi
title_fullStr	Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi
title_full_unstemmed	Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi
title_sort	evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / nurnadirah mat nawi
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2018
url	https://ir.uitm.edu.my/id/eprint/84275/1/84275.pdf
_version_	1794191988651720704

Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi

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