Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem

Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem

Prolonged inflammation and oxidative stress contribute to a plethora of detrimental inflammatory and oxidative diseases. Previous studies have proven Rhodomyrtus tomentosa leaves are well-known anti-inflammatory and antioxidant agent. The present study was conducted to further identify PIC in 95% et...

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Main Author: Ab Keram @ Ab Karem, Azizah Munirah
Format: Thesis
Language:English
Published: 2023
Online Access:https://ir.uitm.edu.my/id/eprint/88731/1/88731.pdf
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id my-uitm-ir.88731
record_format uketd_dc
spelling my-uitm-ir.887312023-12-26T08:07:02Z Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem 2023 Ab Keram @ Ab Karem, Azizah Munirah Prolonged inflammation and oxidative stress contribute to a plethora of detrimental inflammatory and oxidative diseases. Previous studies have proven Rhodomyrtus tomentosa leaves are well-known anti-inflammatory and antioxidant agent. The present study was conducted to further identify PIC in 95% ethanol extracts of R. tomentosa leaves (EtRT) found in Malaysia and assess their cytotoxicity, anti-inflammatory, and antioxidant activities in search of a potential dual-effect drug. Identification of PIC in EtRT extracts was done by utilizing RP- HPLC and LC-MS procedures. Cytotoxicity properties were assessed with the MTT assay while anti-inflammatory properties were assessed by NO inhibition assay and the regulation of iNOS protein expression through the Western Blotting (WB) assay, both in RAW 264.7 macrophage cells. Preliminary antioxidant properties assessment was determined with DPPH radical scavenging activity and FRAP assay, further followed with inhibition of intracellular ROS assay in RAW 264.7 macrophage cells. During RP-HPLC analysis, PIC in EtRT extracts eluted at 4.054 minutes and was confirmed by comparison to its PIC standard peak eluted at 4.028 minutes. 2023 Thesis https://ir.uitm.edu.my/id/eprint/88731/ https://ir.uitm.edu.my/id/eprint/88731/1/88731.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Health Sciences Bahari, Mazura
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Bahari, Mazura
description Prolonged inflammation and oxidative stress contribute to a plethora of detrimental inflammatory and oxidative diseases. Previous studies have proven Rhodomyrtus tomentosa leaves are well-known anti-inflammatory and antioxidant agent. The present study was conducted to further identify PIC in 95% ethanol extracts of R. tomentosa leaves (EtRT) found in Malaysia and assess their cytotoxicity, anti-inflammatory, and antioxidant activities in search of a potential dual-effect drug. Identification of PIC in EtRT extracts was done by utilizing RP- HPLC and LC-MS procedures. Cytotoxicity properties were assessed with the MTT assay while anti-inflammatory properties were assessed by NO inhibition assay and the regulation of iNOS protein expression through the Western Blotting (WB) assay, both in RAW 264.7 macrophage cells. Preliminary antioxidant properties assessment was determined with DPPH radical scavenging activity and FRAP assay, further followed with inhibition of intracellular ROS assay in RAW 264.7 macrophage cells. During RP-HPLC analysis, PIC in EtRT extracts eluted at 4.054 minutes and was confirmed by comparison to its PIC standard peak eluted at 4.028 minutes.
format Thesis
qualification_level Master's degree
author Ab Keram @ Ab Karem, Azizah Munirah
spellingShingle Ab Keram @ Ab Karem, Azizah Munirah
Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem
author_facet Ab Keram @ Ab Karem, Azizah Munirah
author_sort Ab Keram @ Ab Karem, Azizah Munirah
title Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem
title_short Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem
title_full Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem
title_fullStr Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem
title_full_unstemmed Anti-inflammatory and antioxidant properties of ethanol leaf extracts of Rhodomyrtus tomentosa against raw 264.7 macrophage cells / Azizah Munirah Ab Keram @ Ab Karem
title_sort anti-inflammatory and antioxidant properties of ethanol leaf extracts of rhodomyrtus tomentosa against raw 264.7 macrophage cells / azizah munirah ab keram @ ab karem
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Health Sciences
publishDate 2023
url https://ir.uitm.edu.my/id/eprint/88731/1/88731.pdf
_version_ 1794192149313486848

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