Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin

Fungal endophytes produce a broad variety of bioactive compounds with potential to address some of the unmet human needs. Medicinal plants have an important role to play in the search for new strains of endophytes fungi, as it is possible that their beneficial characteristics are as a result of the...

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Main Author:	Mohamed Zanudin, Nur Afeeqah
Format:	Thesis
Language:	English
Published:	2023
Subjects:	QK Botany
Online Access:	https://ir.uitm.edu.my/id/eprint/91013/1/91013.pdf
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id	my-uitm-ir.91013
record_format	uketd_dc
spelling	my-uitm-ir.910132024-07-22T02:58:31Z Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin 2023 Mohamed Zanudin, Nur Afeeqah QK Botany Fungal endophytes produce a broad variety of bioactive compounds with potential to address some of the unmet human needs. Medicinal plants have an important role to play in the search for new strains of endophytes fungi, as it is possible that their beneficial characteristics are as a result of the metabolites produced by their endophytic community. However, inspite of this potential as repositories of bioactive compounds, the fungal endophytes of Malaysian medicinal plants remain largely underexplored. This thesis reports on studies that were conducted to bioprospect for endophytic fungi with antagonistic activity against Collectrichum gloeosporioides hosted by the plants Garcinia atroviridis used in Malaysian traditional medicine. The endophytic fungus isolates were isolated from different parts of the host plant (fruits, leaves and branches), where the samples were collected from two different geographical location in Nasuha Herbs Farm, Muar. A total of 243 endophytic fungi were isolated from 600 G. atrovidis segments. Overall, fungal isolation rate in G. atrovidis segments was 0.41 and colonization rate was 71.5% and the highest being found in the higher location. The colonization rate was higher in the leaf (50.2%) than the branch (33.3%) and fruit (16.5%). The fungal richness was also higher in leaf as compared to in other parts. Morphological analysis grouped these isolates into 21 distinct groups. Further, ITS gene sequencing analysis identified these isolates as Annulohypoxylon sp, Colletotrichum gloeosporioides, Pestalotiopsis sp., Pestalotiopsis sp., Diaporthe sp., Lasiodiplodi theobromae, Pestalotiopsis neglecta, Bjerkandera adusta, Nigrospora sphaerica and Colletotrichum sp. The effect of different cultivation medium on fungal growth revealed that the growth requirements of each endophyte fungi isolate significantly (p<0.05) differs from each other. Potato Dextrose Agar (PDA) exhibited the most suitable medium to growth endophyte fungus with highest radial growth compared to Malt Extract Agar (MAE) and Water Extract Agar (WA). 2023 Thesis https://ir.uitm.edu.my/id/eprint/91013/ https://ir.uitm.edu.my/id/eprint/91013/1/91013.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Hasan, Nor'Aishah Mansor, Patahayah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Hasan, Nor'Aishah Mansor, Patahayah
topic	QK Botany
spellingShingle	QK Botany Mohamed Zanudin, Nur Afeeqah Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin
description	Fungal endophytes produce a broad variety of bioactive compounds with potential to address some of the unmet human needs. Medicinal plants have an important role to play in the search for new strains of endophytes fungi, as it is possible that their beneficial characteristics are as a result of the metabolites produced by their endophytic community. However, inspite of this potential as repositories of bioactive compounds, the fungal endophytes of Malaysian medicinal plants remain largely underexplored. This thesis reports on studies that were conducted to bioprospect for endophytic fungi with antagonistic activity against Collectrichum gloeosporioides hosted by the plants Garcinia atroviridis used in Malaysian traditional medicine. The endophytic fungus isolates were isolated from different parts of the host plant (fruits, leaves and branches), where the samples were collected from two different geographical location in Nasuha Herbs Farm, Muar. A total of 243 endophytic fungi were isolated from 600 G. atrovidis segments. Overall, fungal isolation rate in G. atrovidis segments was 0.41 and colonization rate was 71.5% and the highest being found in the higher location. The colonization rate was higher in the leaf (50.2%) than the branch (33.3%) and fruit (16.5%). The fungal richness was also higher in leaf as compared to in other parts. Morphological analysis grouped these isolates into 21 distinct groups. Further, ITS gene sequencing analysis identified these isolates as Annulohypoxylon sp, Colletotrichum gloeosporioides, Pestalotiopsis sp., Pestalotiopsis sp., Diaporthe sp., Lasiodiplodi theobromae, Pestalotiopsis neglecta, Bjerkandera adusta, Nigrospora sphaerica and Colletotrichum sp. The effect of different cultivation medium on fungal growth revealed that the growth requirements of each endophyte fungi isolate significantly (p<0.05) differs from each other. Potato Dextrose Agar (PDA) exhibited the most suitable medium to growth endophyte fungus with highest radial growth compared to Malt Extract Agar (MAE) and Water Extract Agar (WA).
format	Thesis
qualification_level	Master's degree
author	Mohamed Zanudin, Nur Afeeqah
author_facet	Mohamed Zanudin, Nur Afeeqah
author_sort	Mohamed Zanudin, Nur Afeeqah
title	Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin
title_short	Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin
title_full	Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin
title_fullStr	Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin
title_full_unstemmed	Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin
title_sort	characterization of endophytic fungi from garcinia atroviridis with antagonistic effect against colletotrichum gloeosporioides / nur afeeqah mohamed zanudin
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2023
url	https://ir.uitm.edu.my/id/eprint/91013/1/91013.pdf
_version_	1811768754012422144

Characterization of endophytic fungi from Garcinia atroviridis with antagonistic effect against Colletotrichum gloeosporioides / Nur Afeeqah Mohamed Zanudin

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