Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali

This study presents the potential use of natural product from Allamanda cathartica leaves and stems extract, as biofungicides and their antifungal activity against Pyricularia oryzae pathogen of rice blast disease in vitro and in vivo. The objectives of the study are to observe and evaluate the mech...

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Main Author:	Ali, Khairun Nur
Format:	Thesis
Language:	English
Published:	2018
Online Access:	https://ir.uitm.edu.my/id/eprint/95269/1/95269.pdf
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id	my-uitm-ir.95269
record_format	uketd_dc
spelling	my-uitm-ir.952692024-11-08T03:43:43Z Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali 2018 Ali, Khairun Nur This study presents the potential use of natural product from Allamanda cathartica leaves and stems extract, as biofungicides and their antifungal activity against Pyricularia oryzae pathogen of rice blast disease in vitro and in vivo. The objectives of the study are to observe and evaluate the mechanisms of antifungal activity of A. cathartica leave and stem crude extracts against P. oryzae, pathogen of rice blast disease microscopically and to determine the phytochemical constituent in the A. cathartica leave and stem crude extracts that act as an antifungal activity agent. The results from the experiments revealed that chloroform and methanol stem extracts of A. cathartica possessed fungistatic or fungicidal properties on the growth of P. oryzae. However, chloroform stem crude extract of Allamanda cathartica shows greater inhibition zone (60% inhibition) against P. oryzae compared to methanol stem crude extracts (57.5% inhibition) with the radial growth of 1.6 cm and 1.7 cm respectively. The others crude extracts showed slightly antifungal activity against fungal pathogen. Furthermore, the chloroform and methanol stem crude extracts showed stronger inhibitory action than the positive control, Mancozeb® (41% Inhibition). The effective inhibitory concentration of active crude extract was at 7500 ppm. Microscopic observations of antifungal activity of active extracts resulted in hyphal degradation where hyphae were retarded and lysis. The screening of antifungal activity of fractions of active crude extract showed the good antifungal activity was at fraction 4 (70% hexane: 30% ethanol) with the radial growth inhibition 1.8 cm (55% inhibition). The effectiveness of active crude extract in vivo assays were conducted and found that the curative controls were more effective than preventive control. 2018 Thesis https://ir.uitm.edu.my/id/eprint/95269/ https://ir.uitm.edu.my/id/eprint/95269/1/95269.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology Che Mohd Ramli, Neni Kartini
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Che Mohd Ramli, Neni Kartini
description	This study presents the potential use of natural product from Allamanda cathartica leaves and stems extract, as biofungicides and their antifungal activity against Pyricularia oryzae pathogen of rice blast disease in vitro and in vivo. The objectives of the study are to observe and evaluate the mechanisms of antifungal activity of A. cathartica leave and stem crude extracts against P. oryzae, pathogen of rice blast disease microscopically and to determine the phytochemical constituent in the A. cathartica leave and stem crude extracts that act as an antifungal activity agent. The results from the experiments revealed that chloroform and methanol stem extracts of A. cathartica possessed fungistatic or fungicidal properties on the growth of P. oryzae. However, chloroform stem crude extract of Allamanda cathartica shows greater inhibition zone (60% inhibition) against P. oryzae compared to methanol stem crude extracts (57.5% inhibition) with the radial growth of 1.6 cm and 1.7 cm respectively. The others crude extracts showed slightly antifungal activity against fungal pathogen. Furthermore, the chloroform and methanol stem crude extracts showed stronger inhibitory action than the positive control, Mancozeb® (41% Inhibition). The effective inhibitory concentration of active crude extract was at 7500 ppm. Microscopic observations of antifungal activity of active extracts resulted in hyphal degradation where hyphae were retarded and lysis. The screening of antifungal activity of fractions of active crude extract showed the good antifungal activity was at fraction 4 (70% hexane: 30% ethanol) with the radial growth inhibition 1.8 cm (55% inhibition). The effectiveness of active crude extract in vivo assays were conducted and found that the curative controls were more effective than preventive control.
format	Thesis
qualification_level	Master's degree
author	Ali, Khairun Nur
spellingShingle	Ali, Khairun Nur Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali
author_facet	Ali, Khairun Nur
author_sort	Ali, Khairun Nur
title	Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali
title_short	Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali
title_full	Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali
title_fullStr	Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali
title_full_unstemmed	Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali
title_sort	antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / khairun nur ali
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2018
url	https://ir.uitm.edu.my/id/eprint/95269/1/95269.pdf
_version_	1818587952465313792

Antifungal activity of allamanda cathartica stem and leaf crude extracts against pyricularia oryzae pathogen of rice blast disease / Khairun Nur Ali

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