Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha

The issue regarding the fate of honey products is emerging widely in the market. To differentiate between fate honey and original honey, the chemical and biological profiles of stingless bee honey are essential to fraud and to guarantee the authenticity of the honey. Thus, this study aimed to identi...

Full description

Saved in:

Bibliographic Details
Main Author:	Joha, Siti Khuzaifah
Format:	Thesis
Language:	English
Published:	2022
Subjects:	Physical and theoretical chemistry Analytical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/96269/1/96269.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.96269
record_format	uketd_dc
spelling	my-uitm-ir.962692024-06-07T03:59:36Z Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha 2022 Joha, Siti Khuzaifah Physical and theoretical chemistry Analytical chemistry The issue regarding the fate of honey products is emerging widely in the market. To differentiate between fate honey and original honey, the chemical and biological profiles of stingless bee honey are essential to fraud and to guarantee the authenticity of the honey. Thus, this study aimed to identify the volatile chemical compounds of stingless bee honey using Gas Chromatography-Mass Spectroscopy (GC-MS). In addition, this study assessed the total phenolic and flavonoid content, which were determined spectrophotometrically. Biological activities, such as the antioxidant and antimicrobial activity of the Trigona itama sample, were also assayed using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and minimum inhibitory and bactericidal concentrations assays. The GC spectral data revealed that the major chemical classes found in honey are ether and phenol. One of the major compounds is butylated hydroxytoluene. Besides, 7,15-dihydroxydehydroadiabetic acid and benzene-ethanamine are reported for the first time. The honey sample recorded high total phenolic content of 92.97±1.2 mg GAE/g extract and total flavonoid content of 98.77±0.85 mg QUE/g extract. In addition, the honey samples also exhibited strong antioxidant activities with IC50 of 4.62 μg/mL, comparable with the positive control, quercetin (IC50 = 1.59 μg/mL). The stingless bee honey also demonstrated moderate antimicrobial activity against all the tested bacteria, namely Staphylococcus aureus (SA), Streptococcus pyogenes (SP), Escherichia coli (EC), and Pseudomonas aeruginosa (PA). The findings from this study indicated that stingless bee (Trigona itama) honey could be a strong antioxidant agent and a potent inhibitor of all tested bacteria, SA, SP, EC, and PA. The data obtained from this study will serve as basic information for further product development and increase the commercialization potential. 2022 Thesis https://ir.uitm.edu.my/id/eprint/96269/ https://ir.uitm.edu.my/id/eprint/96269/1/96269.pdf text en public degree Universiti Teknologi MARA, Sarawak Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Physical and theoretical chemistry Analytical chemistry
spellingShingle	Physical and theoretical chemistry Analytical chemistry Joha, Siti Khuzaifah Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha
description	The issue regarding the fate of honey products is emerging widely in the market. To differentiate between fate honey and original honey, the chemical and biological profiles of stingless bee honey are essential to fraud and to guarantee the authenticity of the honey. Thus, this study aimed to identify the volatile chemical compounds of stingless bee honey using Gas Chromatography-Mass Spectroscopy (GC-MS). In addition, this study assessed the total phenolic and flavonoid content, which were determined spectrophotometrically. Biological activities, such as the antioxidant and antimicrobial activity of the Trigona itama sample, were also assayed using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and minimum inhibitory and bactericidal concentrations assays. The GC spectral data revealed that the major chemical classes found in honey are ether and phenol. One of the major compounds is butylated hydroxytoluene. Besides, 7,15-dihydroxydehydroadiabetic acid and benzene-ethanamine are reported for the first time. The honey sample recorded high total phenolic content of 92.97±1.2 mg GAE/g extract and total flavonoid content of 98.77±0.85 mg QUE/g extract. In addition, the honey samples also exhibited strong antioxidant activities with IC50 of 4.62 μg/mL, comparable with the positive control, quercetin (IC50 = 1.59 μg/mL). The stingless bee honey also demonstrated moderate antimicrobial activity against all the tested bacteria, namely Staphylococcus aureus (SA), Streptococcus pyogenes (SP), Escherichia coli (EC), and Pseudomonas aeruginosa (PA). The findings from this study indicated that stingless bee (Trigona itama) honey could be a strong antioxidant agent and a potent inhibitor of all tested bacteria, SA, SP, EC, and PA. The data obtained from this study will serve as basic information for further product development and increase the commercialization potential.
format	Thesis
qualification_level	Bachelor degree
author	Joha, Siti Khuzaifah
author_facet	Joha, Siti Khuzaifah
author_sort	Joha, Siti Khuzaifah
title	Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha
title_short	Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha
title_full	Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha
title_fullStr	Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha
title_full_unstemmed	Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha
title_sort	identification of honey volatile compounds by gas chromatography-mass spectrometry (gc-ms) and its biological activity / siti khuzaifah joha
granting_institution	Universiti Teknologi MARA, Sarawak
granting_department	Faculty of Applied Sciences
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/96269/1/96269.pdf
_version_	1804889981768957952

Identification of honey volatile compounds by Gas Chromatography-Mass Spectrometry (GC-MS) and its biological activity / Siti Khuzaifah Joha

Similar Items