Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir

The purpose of this study was to do an optimization of Reagent B in the newly produced in-house DNA extraction kit, for the purpose of reducing the unnecessary consumption of the reagent but still maintaining the efficiency in extracting an optimum purity and yield of DNA. Subsequently, this extract...

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Bibliographic Details
Main Author:	Md Mahir, Hannah
Format:	Thesis
Language:	English
Published:	2007
Subjects:	DNA Deoxyribonucleic acids RS Pharmacy and materia medica Extraction
Online Access:	https://ir.uitm.edu.my/id/eprint/99961/1/99961.PDF
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id	my-uitm-ir.99961
record_format	uketd_dc
spelling	my-uitm-ir.999612024-08-07T14:15:42Z Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir 2007 Md Mahir, Hannah DNA. Deoxyribonucleic acids RM Therapeutics. Pharmacology RS Pharmacy and materia medica Extraction The purpose of this study was to do an optimization of Reagent B in the newly produced in-house DNA extraction kit, for the purpose of reducing the unnecessary consumption of the reagent but still maintaining the efficiency in extracting an optimum purity and yield of DNA. Subsequently, this extraction kit was tested on several pharma-cosmeceutical products to ensure the existence of porcine DNA traces in the samples. This study was start on by extracting DNA from various samples using the in house DNA extraction kit. Then, followed by conventional PCR to compare the DNA from samples with positive control. Consequently, running gel electrophoresis also being employed after the extraction process to visualize the size of DNA acquired and after the PCR to visualize the size of amplicons produced and to verify the specificity of the primer used. In conclusion, the optimum concentration of Reagent B to be employed in the in-house DNA extraction kit is lmg/ml and the optimized in-house extraction kit plus the primer utilized in this study can become a promising tool in clarification of porcine DNA traces in Halal authentication. 2007 Thesis https://ir.uitm.edu.my/id/eprint/99961/ https://ir.uitm.edu.my/id/eprint/99961/1/99961.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ahmad, Norazlina
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ahmad, Norazlina
topic	DNA Deoxyribonucleic acids DNA Deoxyribonucleic acids RS Pharmacy and materia medica Extraction
spellingShingle	DNA Deoxyribonucleic acids DNA Deoxyribonucleic acids RS Pharmacy and materia medica Extraction Md Mahir, Hannah Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
description	The purpose of this study was to do an optimization of Reagent B in the newly produced in-house DNA extraction kit, for the purpose of reducing the unnecessary consumption of the reagent but still maintaining the efficiency in extracting an optimum purity and yield of DNA. Subsequently, this extraction kit was tested on several pharma-cosmeceutical products to ensure the existence of porcine DNA traces in the samples. This study was start on by extracting DNA from various samples using the in house DNA extraction kit. Then, followed by conventional PCR to compare the DNA from samples with positive control. Consequently, running gel electrophoresis also being employed after the extraction process to visualize the size of DNA acquired and after the PCR to visualize the size of amplicons produced and to verify the specificity of the primer used. In conclusion, the optimum concentration of Reagent B to be employed in the in-house DNA extraction kit is lmg/ml and the optimized in-house extraction kit plus the primer utilized in this study can become a promising tool in clarification of porcine DNA traces in Halal authentication.
format	Thesis
qualification_level	Bachelor degree
author	Md Mahir, Hannah
author_facet	Md Mahir, Hannah
author_sort	Md Mahir, Hannah
title	Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_short	Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_full	Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_fullStr	Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_full_unstemmed	Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_sort	optimizing in-house dna extraction system for the detection of sus scrofa dna traces in pharma-cosmeceutical product / hannah md mahir
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2007
url	https://ir.uitm.edu.my/id/eprint/99961/1/99961.PDF
_version_	1811769066300375040

Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir

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